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Plants offer an alternative inexpensive and convenient technology for large scale production of recombinant proteins especially recombinant antibodies (plantibodies). In this paper, we describe the expression of a model single chain antibody fragment (B6scFv) in transgenic tobacco. Four different gene constructs of B6scFv with different target signals for(More)
Transcript profiling and immunodetection of MAPK4 and MAPK6 proteins were carried out at different stages of Alternaria blight disease in Varuna (susceptible) and PAB 9511 (tolerant) genotypes of Brassica juncea to probe the role of these kinases during pathogenesis. The transcripts of both MAPK4 and MAPK6 correlated with their protein products at different(More)
Immunoglobulin G4 (IgG4-related diseases) affects various tissues and organs of the human body. Orbital, adnexal, and scleral inflammations were already reported in the medical literature. To the best of our knowledge, we report the first case of intraocular IgG4-associated inflammatory mass in the ciliary body mimicking as a melanoma in a 23-year-old(More)
Quantification of greenhouse gas (GHG) emissions from agricultural lands is essential for strategic planning towards GHG efficient development in India. We measured the fluxes of CH4, N2O and CO2 during cultivation of two important crops; sugarcane and pigeon pea following the closed chamber technique. Both the soils acted as net CH4 sinks, but sources of(More)
Random amplified polymorphic DNA-PCR (RAPD-PCR) analysis was used in combination with morphological characters to investigate genetic variations and population structure of Callosobruchus maculatus and Callosobruchus chinensis species from Bundelkhand region of India. It has proved its usefulness to get information quickly in comparatively less-studied(More)
Performance of a manufacturing system depends on the performance at the shopfloor level. Traditionally, shopfloor level operational policies concerning maintenance, quality and production-scheduling have been considered and optimized independently. However, these three aspects of operations planning may also have an interaction effect on each other and(More)
Human myiasis refers to parasitic infestation of body tissues by larvae of several fly species. The entity has a simple management. Human myiasis is well-documented in the literature however genital myiasis in females is scarcely reported in the literature. We hereby report this entity in an adolescent female who presented with urinary retention and(More)
Electrophoretic separation of humic acids obtained from soil was attempted through denaturing polyacrylamide gel electrophoresis (dn-PAGE) by modifying the known methods. The modifications of sample preparation and gel composition led to significant improvement in both visibility and intensity of humic acid profile. The comparative profile showed a similar(More)
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