Dirk Költgen

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1. Pulses of acetylcholine (ACh) in concentrations between 0.1 and 1000 microM were applied repetitively to outside-out patches of enzymatically denervated (14 days) mouse muscle with the liquid filament switch. Solutions superfusing the patch could be changed rapidly (within 0.2 ms). 2. Single-channel activity was studied under steady-state conditions in(More)
To assess the postsynaptic consequences of botulinum toxin injection into muscle we characterized the nicotinic acetylcholine receptor (nAChR) with the patch clamp technique, using adult mouse muscle after destruction of the nerve ending and after treatment with botulinum toxin (BoTX). In both, embryonic channels with a conductance of 30 and 34 pS could be(More)
In the sarcolemma of innervated dystrophic mdx mouse muscle we found the embryonic and the adult nicotinic acetylcholine receptor, where normally after innervation only the adult type would be expected, as can be shown with comparative data from patch-clamp experiments from wildtype and wildtype-denervated muscle. As a control to the dystrophic muscle we(More)
Dissociated interosseus muscle fibres of wildtype and mdx mice were investigated to characterize acetylcholine (ACh) receptors with the single channel recording patch-clamp technique. On the muscle fibres of mdx mutants, two types of nicotinic acetylcholine receptor (nAChR) channels were found. One channel (29 pS, mean open time 2.1 ms) resembles channels(More)
A linear Cl− channel with a mean conductance of 16.3±0.4 pS (n=7), as determined for inwardly directed currents in the cell-attached mode, was identified by the patch clamp technique in the apical membrane of Caco-2 cells in the early stage of postconfluential differentiation. This channel exhibited a low spontaneous activity (in 3 out of 137 experiments)(More)
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