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Serum samples from 51 apparently healthy breeding bulls were screened for bovine herpesvirus-1 (BHV-1) antibodies using an avidin-biotin enzyme-linked immunosorbent assay, revealing a sero-positive prevalence rate of 45.09%. Semen samples were then collected from 12 of the sero-positive and 12 of the sero-negative bulls and tested for BHV-1 antigen using(More)
Staphylococcus aureus is a Gram-positive bacterium that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to need for an alternative treatment such as bacteriophage therapy. Present study describes isolation and characterization of(More)
Mesenchymal stem cells are multipotent stem cells capable of differentiating into various cell types, including osteocytes, chondrocytes, adipocytes, myocytes, and tenocytes. They are being highly investigated in tissue engineering and regenerative studies. This study was carried out to isolate, identify and examine the differentiation potential of canine(More)
AIM This study was aimed at evaluating the anti-apoptotic effects of Bcl-2 protein in cryopreserved buffalo bull sperm. MATERIALS AND METHODS A total 10 ejaculates from two buffalo bulls (5 each) were collected using artificial vagina method, and semen was evaluated using a standard protocol. Semen was extended by Tris egg yolk extender supplemented with(More)
Brucellosis is a disease caused by Brucella sp which is an aerobic, non-spore forming, non-motile gram negative coccobacillus. It is a National Institute of Health category B bioterror threat agent that causes incapacitating zoonosis. It is an emerging zoonosis, and an economically important infection of humans and livestock with a worldwide distribution.(More)
  • M Dandale, C K Singh, +5 authors Guru Angad Dev
  • 2012
TaqMan PCR was employed for ante mortem diagnosis of rabies from 62 biological fluid (24 saliva, 17 milk, 21urine) samples obtained from 25 animals suspected for rabies. Rabies viral RNA was detected in 12/24 (50%) saliva samples, 4/17 (23.52%) milk samples and 11/21 (52.38%) urine samples. Rabies was detected with highest sensitivity of 78.94% from urine(More)
The data described are related to the article entitled "Sequence Analysis of Meq oncogene among Indian isolates of Marek׳s Disease Herpesvirus" M. Gupta, D. Deka, Ramneek, 2016. Seven meq genes of Ludhiana Marek׳s disease virus (MDV) field isolates were PCR amplified by using proof reading Platinum Pfx DNA polymerase enzyme, sequenced and then analyzed for(More)
Hepatitis E virus (HEV) has two discrete epidemiological patterns: waterborne epidemics in developing countries only, caused by HEV genotype I, and sporadic zoonotic outbreaks in developing and developed countries caused by genotypes III and IV. This study was designed to investigate seroprevalence, molecular detection and the characterization of HEV by(More)
Canine distemper (CD), caused by canine distemper virus (CDV) is a highly contagious disease that infects a variety of carnivores. Sequence analysis of CDVs from different geographical areas has shown a lot of variation in the genome of the virus especially in haemagglutinin gene which might be one of the causes of vaccine failure. In this study, we(More)
The present investigation was conducted to study the genetic heterogenicity and molecular polymorphism among the field isolates and vaccine strains of infectious bursal disease virus (IBDV). Samples of bursa of Fabricius from 15 suspected outbreaks of infectious bursal disease (IBD) were subjected to agar gel precipitation test (AGPT), virus isolation and(More)