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  • Dimitris N. Papageorgiou, Jeroen A. A. Demmers, John Strouboulis
  • Biology, Medicine
  • Protein expression and purification
  • 2013 (First Published: 1 May 2013)
  • We describe here a simple procedure for greatly reducing contamination of nuclear extracts by naturally biotinylated cytoplasmic carboxylases, which represent a major source of non-specificContinue Reading
  • Dimitris N. Papageorgiou, Elena Karkoulia, +6 authors John Strouboulis
  • Biology, Medicine
  • Biochimica et biophysica acta
  • 2016 (First Published: 1 December 2016)
  • DNMT1 is the maintenance DNA methyltransferase shown to be essential for embryonic development and cellular growth and differentiation in many somatic tissues in mammals. Increasing evidence has alsoContinue Reading
  • Marina Ioannou, Dimitris N. Papageorgiou, Vasily Ogryzko, John Strouboulis
  • Medicine
  • BMC research notes
  • 2018 (First Published: 1 December 2018)
  • AbstractΟbjectiveTo construct mammalian expression vectors for the N- or C-terminal tagging of proteins with a tandem affinity tag comprised of the biotinylatable Avi tag and of a triple FLAGContinue Reading