Dik Harbrecht

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E. coli lysogenic for the temperate, lambda-related phage HK022 do not support lambda growth. The exclusion of lambda is caused by the HK022 nun gene product, which blocks the expression of genes located downstream of and in the same transcription unit as the lambda nutL and nutR sequences. Transcripts terminating prematurely at or near nutR have been(More)
A vector system has been designed for obtaining high yields of polypeptides synthesized in Escherichia coli. Multiple copies of a synthetic gene encoding the neuropeptide substance P (SP) (Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2) have been linked and fused to the lacZ gene. Each copy of the SP gene was flanked by codons for methionine to create(More)
  • Johan W A Traa, Eric Logicacmg, +4 authors Mentor Logicacmg
  • 2006
3 4 ABSTRACT For this thesis, which I write for the study Informatics & Economics at the Erasmus University Rotterdam, The Netherlands, I am a trainee at LogicaCMG Rotterdam. There are numerous ways for a software development organisation to create an information technology solution for the customer. All go through a multistep procedure from analysis of the(More)
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