Dietrich Wolter

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At the present time there is an enormously increasing demand for albumin. The most common procedure for the isolation of this plasma component is the cold ethanol technique developed by Cohn. Because this process necessarily isolates other blood components for which there is less demand in relation to albumin, albumin production is expensive. Therefore, we(More)
1. Prostanoid synthesis was induced in bone marrow-derived macrophages by addition of exogenous arachidonic acid to the cell cultures. When the cells were preincubated with dexamethasone (10(-7) and 10(-6) M) overnight, prostaglandin synthesis was inhibited by 66.5 +/- 2.8% and 56.7 +/- 2.9% (mean +/- s.d.; n = 3) respectively. 2. Endogenous membrane bound(More)
Injection of endotoxin in D-galactosamine sensitized mice resulted in increased serum levels of tumor necrosis factor as determined in a fibroblast cytolysis assay. Concomitant injection of different lipoxygenase inhibitors decreased the titer of TNF. When the lipoxygenase inhibitors were tested in macrophage cultures stimulated with LPS, they prevented the(More)
Although the heat-ethanol method of fractionation for the isolation of normal serum albumin (NSA) is technically easier and more economic than cold-ethanol methods, globulin removal and albumin concentration remain basic hinderances to a truly simplified procedure. Continuous flow centrifugation for the separation of precipitated proteins has been(More)
Oxpentifylline (pentoxifylline), which is known to have pharmacological effects in animal models of respiratory distress syndrome, multiorgan failure, and shock, was tested in human beings after injection of endotoxin. Of ten healthy volunteers, nine met the inclusion criterion of a rise in body temperature of at least 1.0 degrees C after 100 ng endotoxin(More)
Mononuclear phagocytes convert arachidonic acid and other unsaturated fatty acids from intracellular sources to a variety of oxygenated metabolites such as prostaglandins and leukotrienes which are secreted into the surrounding medium. Other oxidative products such as hydroxylinoleic acids are reacylated into cellular constituents. The underlying metabolic(More)
Albumin isolated by the Hagen/Münster heat-ethanol method is a remarkably stable solution of high purity and clinical compatibility. The simplicity of this method makes it a model fractionation procedure for the small transfusion service. Immunization studies carried out by us are compared with previous accounts of a "new" antigen occuring in plasma protein(More)
The availibility of a non-modified gamma globulin for intravenous use should abolish the intramuscular administration of Cohn standard gamma globulin and replace modified products to the greatest possible extent. The product developed in our institute is purified from Cohn fraction II using HES and aluminum silicate for the elimination of aggregates. The(More)
In a comparative study a total volume of 1435 kg outdated freeze-dried plasma, equivalent to approx. 200,000 kg liquid plasma, was fractionated into albumin (20%): about 30% of the total plasma volume was fractionated following the cold-ethanol procedure and about 70% following the heat-ethanol method. Average albumin recovery following cold-ethanol(More)
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