Diego Infante

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A cDNA of the sea urchin Strongylocentrotus purpuratus was identified as encoding polyubiquitin and used to detect a single gene with transcripts containing multiple ubiquitin coding units. Polyubiquitin transcripts exist as a 3.2-kb RNA in polyribosomes and as three higher molecular weight RNAs in purified nuclei. The amount of polyubiquitin RNA is(More)
Borrelia hermsii, a relapsing fever agent, undergoes multiphasic antigenic variation to evade its host's immune response. Serotype specificity is determined by variable membrane lipoproteins, Vmps, which are expressed from genes located near the end of a linear plasmid. Using the polymerase chain reaction and primers representing the promoter of the active(More)
A large fraction of the translationally repressed non-globin messenger RNA in duck erythroblasts is present in non-polyribosomal free mRNP structures which sediment in the 30-40-S range ('35 S'). In 0.5 M KCl, they form core complexes which show a pronounced peak at about 32 S containing mRNA and a discrete spherical RNP particle with a diameter of about 12(More)
Wild tomato plants of Lycopersicon peruvianum var. dentatum were transformed with pG11 plasmid having human beta-interferon (hIFN-beta) and NPTII genes. Transformation was demonstrated by polymerase chain reaction (PCR) and ELISA assay. Expression of hIFN-beta gene was identified by Western blot analysis. Transgenic plants produced seeds and F1 generation(More)
We have used three mammalian in vitro assays for translational initiation (globin synthesis, methionyl-puromycin synthesis, and ternary complex formation), consisting of defined components, to ask whether sea urchin (Strongylocentrotus purpuratus) egg and embryo translational components are active in heterologous assays for mammalian components, and to(More)
The aim of this paper is to present the development of a semi-automatic segmentation method for human knee cartilage and bones from magnetic resonance images (MRI). This segmentation process belongs to a higher aim that consists of a 3D simulation of knee joint to find how stresses are distributed in contact regions between cartilages and to quantify them(More)
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