Learn More
Plants employ post-transcriptional gene silencing (PTGS) as an antiviral defense response. In this mechanism, viral-derived small RNAs are incorporated into the RNA-induced silencing complex (RISC) to guide degradation of the corresponding viral RNAs. ARGONAUTE1 (AGO1) is a key component of RISC: it carries the RNA slicer activity. As a counter-defense,(More)
RNA silencing is a manifestation of a ubiquitous phenomenon that acts, at least in plants and some insects, as a natural defense mechanism against viruses. As a counter-strategy, viruses have evolved to encode silencing suppressor proteins (SSPs) that can block the defense response and evade the host immunity. Although numerous SSP have been identified,(More)
To counteract plant defence mechanisms, plant viruses have evolved to encode RNA silencing suppressor (RSS) proteins. These proteins can be identified by a range of silencing suppressor assays. Here, we describe a simple method using beet necrotic yellow vein virus (BNYVV) that allows a rapid screening of RSS activity. The viral inoculum consisted of BNYVV(More)
  • 1