Dhirendra N. Misra

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In the rat homologue of the mouse Q/TL region, grc-G/C, a TL-like gene (RT1.N) has been identified recently. This paper reports on a Q-like gene, designated RT1.0, that maps in the same region. It contains a 5' untranslated region (UT), signal peptide, alpha 3 domain, transmembrane region, cytoplasmic domain (three exons) and 3'UT region. Comparison with(More)
Gastric parietal cells have been accepted as the only site of intrinsic factor production in the human stomach. In animals, however, intrinsic factor has been localised to various other cell types of foregut origin, including chief and enteroendocrine cells in gastric mucosa, and duct cells from salivary glands and pancreas. The availability of recombinant(More)
Monoclonal antibodies (mAb) were made against class I MHC antigens of the i (mAb 42,70,39) and u (mAb 68-D) haplotypes in the rat by using specific strain combinations in order to obtain reagents for identifying the products of the RT1.An, RT1.Au, and RT1.Eu loci. These antibodies were hemagglutinating only; were IgG except for mAb 68-D3, which had a(More)
The antibody response to the semiallogeneic fetus in the rat was examined in eight mating combinations that were chosen specifically to cross different major histocompatibility complex (MHC) and nonMHC loci. The BN X DA and BN X BN.1A(DA) matings (n anti-a) were the only ones to show an antibody response to the fetus, and the response occurred in 25-64% of(More)
Isolated plasma membranes of thymic and splenic lymphocytes from unimmunized and immunized rats of the inbred ACI and F344 strains were analyzed for chemical and enzymatic composition, for membrane protein patterns by polyacrylamide gel electrophoresis and for membrane-associated immunoglobulins. After immunization, the thymic and splenic lymphocyte(More)
Efforts were made to generate hybridomas producing monoclonal antibodies against the RT2a, RT2b and RT3a antigens of the rat. While a number of hybridomas from each of five different fusions was found to produce antibodies against the RT2b antigen, no hybridoma producing antibody to the RT2a antigen could be detected among those generated in six different(More)
In some mating combinations in rats, there is a maternal antibody response to the maternal antigenic components of the placenta without any previous immunization of the mother. The highest response occurs in the WF (u) female mated to the DA (a) male, and it is against a unique MHC-encoded class I antigen, the Pa antigen, and not against the major(More)
The expression and degree of amplification of the MYCN oncogene in neuroblastoma provide an important indicator of disease prognosis. Detection of MYCN amplification has been described using Southern blotting or polymerase chain reaction (PCR) on DNA from fresh or frozen tissue samples, and using in situ hybridization mainly on metaphase spreads or smears(More)
Glycoproteins and lipids of rat pancreatic acinar cell carcinomas maintained in nude mice and in cell culture, were analyzed. The tumor contained significantly elevated levels of glycoproteins when compared with their normal counterparts. SDS-PAGE of tumor glycoproteins revealed that there were increased amounts of small molecular weight glycoproteins and(More)