Derrell Chapman

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We have recently developed a novel molecular technique for identification of specific bacterial species within a complex mixture. The technique uses PCR to amplify small subunit ribosomal RNA (SSU rRNA) genes from a mixture of bacteria. One of the PCR primers is labeled with a fluorescent dye to allow detection of the amplified product. The PCR product is(More)
Differential scanning calorimetry has been applied to the study of some simple model biomembranes, i.e. lipid-water systems, and the endothermic phase transitions which they exhibit when transforming from the gel to liquid crystal phase have been studied. These studies show that: 1. When lipids of the same class, e.g. lecithins with dissimilar chain lengths(More)
IcmF is a 5'-deoxyadenosylcobalamin (AdoCbl)-dependent enzyme that catalyzes the carbon skeleton rearrangement of isobutyryl-CoA to butyryl-CoA. It is a bifunctional protein resulting from the fusion of a G-protein chaperone with GTPase activity and the cofactor- and substrate-binding mutase domains with isomerase activity. IcmF is prone to inactivation(More)
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