Derek R Pollard

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Eight pairs of synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) protocol to detect genes for staphylococcal enterotoxins A to E, exfoliative toxins A and B, and toxic shock syndrome toxin 1 in Staphylococcus aureus strains isolated from clinical specimens and contaminated foods. Primers were targeted to internal regions of(More)
A set of four synthetic oligonucleotide probes derived from sequences of the VT1 (Shiga-like toxin I [SLT-I]) and VT2 (SLT-II) genes were used in a polymerase chain reaction (PCR) amplification procedure to detect these genes in some enteric pathogens. A total of 40 verotoxin-producing Escherichia coli strains and 43 isolates of other recognized enteric(More)
Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) technique to detect the gene for aerolysin in strains of Aeromonas hydrophila and to screen for identical genes in A. caviae, A. sobria, and A. veronii isolated from patients with diarrheal disease. Primers targeted a 209-bp fragment of the aer gene coding for the(More)
Two sets of synthetic oligonucleotide primers were used in a polymerase chain reaction adaptation to distinguish the closely related genes for type 2 verotoxin (VT2 or Shiga-like toxin [SLT-II]) and the verotoxin associated with porcine edema disease (VTe or SLT-II variant [SLT-IIv]) in Escherichia coli.
A six-stage stereoselective synthesis of indanyl-7-(3'-pyridyl)-(3R,6R,7R)-2,5-diketopiperazines oxytocin antagonists from indene is described. SAR studies involving mono- and disubstitution in the 3'-pyridyl ring and variation of the 3-isobutyl group gave potent compounds (pK(i) > 9.0) with good aqueous solubility. Evaluation of the pharmacokinetic profile(More)
It is likely that individuals with nonspecific LBP (nsLBP) constitute a heterogenic group and targeting treatment appropriately to those most likely to respond is of major relevance. The STarT Back Tool (SBT) has been developed to stratify patients into risk groups to aid management choices. However, there is controversy over its generalisability and(More)
Mouse embryos exposed to concentrations of 5-bromodeoxyuridine (BUdR) ranging from 0-01 to 1-0 mug/ml in vitro for two days from the 8-cell stage exhibit a concentration-dependent decrease in the frequency of normal blastocysts and decrease in average cell number per embryo. A 20-h exposure was adequate to achieve the full BUdR response. Both effects were(More)
The polymerase chain reaction is an in vitro procedure for primer-directed enzymatic amplification of specific template nucleic acid sequences. This technique was used to detect and differentiate Chlamydia trachomatis and Chlamydia psittaci in laboratory samples of infected McCoy cells. The polymerase chain reaction was shown to be both sensitive, detecting(More)
Sera from BB Wistar rats and Wistar control rats were evaluated for the presence of islet cell antibodies in a prospective study using an indirect immunofluorescence assay on pancreatic islet cell suspensions from cultured rat islets. Islet cell surface antibodies were detected in sera from all animals of the spontaneously diabetic BB Wistar rat colony. The(More)