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When Xenopus eggs and egg extracts replicate DNA, replication origins are positioned randomly with respect to DNA sequence. However, a completely random distribution of origins would generate some unacceptably large interorigin distances. We have investigated the distribution of replication origins in Xenopus sperm nuclei replicating in Xenopus egg extract.(More)
Salinity is a major abiotic stress that limits plant productivity. Plants respond to salinity by switching on a coordinated set of physiological and molecular responses that can result in acclimation. Medicago truncatula is an important model legume species, thus understanding salt stress responses and acclimation in this species is of both fundamental and(More)
A LEAFY-like gene was cloned in the long-day (LD) plant, Silene coeli-rosa (ScLFY). The open reading frame spans 1452 bp encoding a putative protein of 483 amino acids. Amino acid homology to other LFYs is 43-55%; conserved and variable regions were similar to others. However, an intron (808 bp) not found in others occurred close to the N-terminal of the C1(More)
BACKGROUND AND AIMS In published studies, positive relationships between nucleotype and the duration of the mitotic cell cycle in angiosperms have been reported but the highest number of species analyzed was approx. 60. Here an analysis is presented of DNA C-values and cell cycle times in root apical meristems of angiosperms comprising 110 measurements,(More)
In yeasts and animals, premature entry into mitosis is prevented by the inhibitory phosphorylation of cyclin-dependent kinase (CDK) by WEE1 kinase, and, at mitosis, WEE1 protein is removed through the action of the 26S proteasome. Although in higher plants WEE1 function has been confirmed in the DNA replication checkpoint, Arabidopsis wee1 insertion mutants(More)
In animals, 14-3-3 proteins bind two cell cycle proteins WEE1 and CDC25 stabilising their phosphorylated state. We report here for the first time interactions between WEE1 and 14-3-3 proteins both in vitro and in vivo in plants. The Arabidopsis 14-3-3 family partitions into either an Epsilon or Non-Epsilon group. In a yeast 2-hybrid screen Arabidopsis WEE1(More)
Cytogenetic analysis of a 4 year old girl with developmental delay and dysmorphic features showed extra chromosomal material of unknown origin on 20p (46,XX,add(20)(p13)). Familial chromosome studies showed direct inheritance of add(20)(p13) from the father, who had a similar, albeit milder, phenotype. Fibroblast chromosome studies of the father showed no(More)
Xyloglucan oligosaccharides (XGOs) are breakdown products of XGs, the most abundant hemicelluloses of the primary cell walls of non-Poalean species. Treatment of cell cultures or whole plants with XGOs results in accelerated cell elongation and cell division, changes in primary root growth, and a stimulation of defence responses. They may therefore act as(More)
If, following an inductive treatment of 2 d of continuous darkness, shoot apices of Pharbitis nil are cultured 1 d later on White's medium supplemented with 2% sucrose, they cannot form carpels, but they can if they are cultured on 2% glucose. It was hypothesized that the differential effect of these sugars was because of differential expression of(More)
The aim was to test promotive effects of oligosaccharins on root growth and development at the root apical meristem and the cell cycle using the model systems, Arabidopsis thaliana and the tobacco (Nicotiana tabacum) BY-2 cell line. Arabidopsis was grown on medium supplemented with 0.1 mg L−1 oligoxyloglucan (OX), 10 mg L−1 Pectimorf® (P) or 0.5 mg L−1(More)