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We have developed an injection technique which simplifies the use of fluorescent tracers in investigating the projections of peripheral nerves both in rats and pigeons, and avoids the difficulties experienced with the cut nerve technique. The tracers are injected into the peripheral nerve and transported to cell bodies in the dorsal root ganglia (DRG). By a(More)
We have investigated the possibility of somato-visceral convergence in thoracic sensory dorsal root ganglia (DRG) of cats, using the technique of double-labelling with fluorescent tracers. Combinations of propidium iodide (PI) and Fast Blue (FB) or bisbenzimide (Bb) were found to be the most appropriate. FB or Bb were injected into the splanchnic nerve, and(More)
In rabbits and rats under urethane anesthesia, a systematic survey was made of the caudal trigeminal nucleus, using glass coated tungsten microelectrodes. This revealed many neurons in the marginal layer sensitive either to warming or cooling the facial skin. The majority of these neurons were specifically temperature sensitive. In rats, a somatotopic(More)
The somatosensory projection to the striatum of the rat was investigated by electrophysiological mapping. Fifty-five units were encountered that responded to specific forms of stimulation delivered to particular parts of the body. Units that responded to stimuli delivered to the hindlimb, scrotum and tail were found in the most caudal regions of the(More)
The projection of peripheral sensory and motor nerves was investigated in the pigeon (Columba livia) by means of retrogradely transported fluorescent dyes. Two combinations of fluorescent tracers were used that could be identified within the same cell when excited by light of 405 nm: 1) Propidium iodide and Bisbenzimide, which label the cytoplasm orange and(More)
1. Single unit activity has been recorded in the thalamic, hypothalamic and raphe magnus nuclei of rats anaesthetized with Urethane.2. Neurones were sought which responded to changes in scrotal skin temperature applied with a water-perfused brass thermode. All sixty-nine neurones in the thalamus and hypothalamus responded with abrupt changes in activity as(More)
The technique of double labelling with fluorescent tracers has been used to demonstrate that some sensory neurones in the sixth lumbar dorsal root ganglion (L6) of the rat possess peripheral processes which dichotomize into both the pudendal and sciatic nerves. Bisbenzimide (Bb) was injected into the pudendal nerve and propidium iodide (PI) into the(More)
To achieve the ultimate goal of attribute-based access control (ABAC), a robust architecture for Identity, Credential, and Access Management must first be established. The National Aeronautics and Space Administration (NASA) began formal development of its Identity, Credential, and Access Management Architecture using the Zachman Framework for Enterprise(More)
Capsaicin injected into the scrotal skin of rats was observed to induce a decrease in the amount of horseradish peroxidase (HRP) transported in the pudendal nerve to the sixth lumbar dorsal root ganglion on the pretreated side. This was seen as a decrease in the number of HRP-labelled neurones compared to the control side. A morphometric study confirmed(More)