Denis Nadeau

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The addition of 1 part of 1.5 M NaCl or 2.5 M sucrose (10 X concentrate) to 9 parts of Percoll produces a stock solution that is hypertonic (350-360 mOsm/kg H2O). Because the osmolality is a critical variable in the isopycnic separation of cells and cell organelles, the factors accountable for this hypertonicity were investigated. Percoll, a colloidal(More)
A recombinant form of hirudin (HIR), a potent thrombin inhibitor derived from the leech Hirudo medicinalis, was cloned and expressed in the methylotrophic yeast Pichia pastoris. The HIR gene was inserted into the P. pastoris pPic9K expression vector such that the gene's expression is under alcohol oxidase (AOX1) promoter control and the HIR coding sequence(More)
OBJECTIVES To describe the timing of onset of organ system failure, multiple organ system failure diagnosis, and the subsequent death in children admitted to a pediatric intensive care unit (ICU). Second, to identify independent risk markers of death in pediatric patients with multiple organ system failure. DESIGN Review of a database. SETTING Pediatric(More)
The poly(ADP-ribose) polymerase (PARP-1), a 113 kDa nuclear enzyme, is cleaved in fragments of 89 and 24 kDa during apoptosis. This cleavage has become a useful hallmark of apoptosis and has been shown to be done by DEVD-ase caspases, a family of proteases activated during apoptosis. Interestingly, PARP-1 is also processed during necrosis but a major(More)
Coelomocytes were extruded from three earthworm species: Lumbricus terrestris, Eisenia fetida and Octolasion tyrtaeum. Featuring a simple low-vacuum holding device, the proposed methodology allows the recovery of cells with minimum risk of contamination by faecal material. The viability of O. tyrtaeum coelomocytes was highly reproducible (average 93%), with(More)
The biological reactivity of ambient air particles was studied in five in vitro lung macrophage assays, involving the release of cytoplasmic and lysosomal enzymes, cellular ATP, neutral red uptake, tetrazolium reduction, and chemiluminescence. Macrophages from rat lungs (2 x 10(5) cells; 1 cm(2) attachment surface; 1 ml culture medium) were exposed for 18(More)
To determine the incidence of infection secondary to arterial catheterization in children as well as the risk markers, we prospectively evaluated, during a 1-year period, all arterial catheters installed in children admitted to the pediatric intensive care unit. A total of 340 cannulas were placed in 310 children aged 80 +/- 4 months (mean +/- SEM) for a(More)
The membranolytic and cytotoxic properties of two naturally occurring (chrysotile asbestos; attapulgite clay) and two man-made (Fiberfrax, an aluminium-silicate, and xonotlite, a calcium silicate) industrial minerals were compared. "Short" fiber fractions of chrysotile and Fiberfrax were obtained by sedimentation in demineralized water, while the(More)
Aramid fibres have been proposed as a substitute for many asbestos uses. Although dimensional characteristics of aramid fibres vary with the type of application, some of the commercial grades proposed contain fibres whose geometry is clearly in a range where biological reactivities have been reported for other natural or man-made fibres. We wish to report(More)
Meconium in labor is associated with increased perinatal morbidity and mortality. To identify the infants at risk, 53 women with moderate-to-thick meconium were followed in labor after obtaining baseline fetal scalp blood pH levels. Although 28 of the newborns (53%) exhibited an arterial cord pH of less than 7.25 at delivery, there were no significant(More)