Denis N. Khatri

  • Citations Per Year
Learn More
This work was designed to explore efficacy of apelin-12 (A-12) as a cardioprotective agent when given before ischemia or at reperfusion using the isolated working heart model. Hearts of male Wistar rats were subjected to 30-min stabilization period followed by 35-min global ischemia and 30-min reperfusion. A short-term infusion of Krebs-Henseleit buffer(More)
OBJECTIVE To examine cardioprotective effects of Ρ-terminal fragment of adipokine apelin-12 (A12), its novel structural analogue [MeArg(1), NLe(10)]-A12 (I), and [d-Ala(12)]-A12 (II), a putative antagonist of APJ receptor, employing in vivo model of ischemia/reperfusion (I/R) injury. MATERIALS AND METHODS Peptides were synthesized by the automatic solid(More)
Apelin-12 (A-12) peptide was synthesized by automated solid phase method and purified by reverse phase HPLC. Its homogeneity and structure were confirmed by HPLC, 1H-NMR spectroscopy, and mass spectroscopy. Acute myocardial infarction was induced by 40-min occlusion of the left coronary artery with subsequent 60-min reperfusion in narcotized Wistar rats.(More)
The adipocytokine apelin peptide, which consists of 77 amino acid residues, and its APJ receptor con nected with G proteins of membranes of endothelial cells and cardiomyocytes participate in regulation of the tone of coronary blood vessels and contractility of the myocardium [1]. C terminal fragments of the pep tide (apelin 12, 13, and 36) improve the(More)
  • 1