Demetri M. Kokkinakis

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/v-Nitroso-2,6-dimethylmorpholine (NNDM) is an indirect ni-trosamine carcinogen which induces ductal adenocarcinoma of the pancreas in the Syrian golden hamster. NNDM is oxidized by normal male hamster pancreas postmitochondrial superna-tants (S-9) in the presence of reduced nicotinamide adenine dinucleotide phosphate and oxygen to give three major identi(More)
Methionine (MET)-dependent cell lines require MET to proliferate, and homocysteine (HCY) does not act as a substitute for this requirement. From six O6-methylguanine-DNA methyltransferase (MGMT)-efficient (mer+) cell lines tested, two medulloblastomas (Daoy and D-341) and a lung non-small-cell adenocarcinoma with metastatic potential (H-1623) were most(More)
Rats and hamsters were administered a single dose of N-[1-14C]nitroso(2-hydroxypropyl)(2-oxopropyl)amine (HPOP), and their urinary metabolites were examined at various time intervals. In both species, urinary excretion of radiolabeled metabolites reached a plateau at 6 h following injection. At this time, 35 and 28% of the total dose was found in the urine(More)
Liver preparations from Syrian golden hamsters catalyze the metabolism of the pancreatic carcinogen N-nitroso-2,6-dimethylmorpholine largely to N-nitroso(2-hydroxypropyl)(2-oxopropyl)amine (HPOP). This reaction is catalyzed by a mixed-function oxidase in the presence of reduced nicotinamide adenine dinucleotide phosphate and oxygen at a rate of 3.8(More)
The c/s isomer of A/-nitroso-2,6-dimethylmorpholine (NNDM), a pancreatic carcinogen for the Syrian golden hamster, is metab olized by hamster liver microsomes to yield A/-nitroso(2-hy-droxypropylX2-oxopropyl)amine (HPOP) as the major product. Rabbit liver microsomes catalyze the metabolism of c/s-NNDM to HPOP at a rate slower than that observed with hamster(More)
N-Nitroso(2-hydroxypropyl)(2-oxopropyl)amine (HPOP) is a complete pancreatic carcinogen in female hamsters at a dose of 210 mg/kg given via an Alzet 2001 pump implanted s.c. Ultimate carcinogenic metabolites of HPOP target DNA to yield 7-and O6-methylguanines (7-mGua, O6-mGua) and 7- and O6-hydroxypropylguanines (7-HpGua, O6-HpGua). During continuous(More)
/v-Nitroso-2,6-dimethylmorpholine (NNDM) is an indirect ni-trosamine carcinogen which induces ductal adenocarcinoma of the pancreas in the Syrian golden hamster. NNDM is oxidized by normal male hamster pancreas postmitochondrial superna-tants (S-9) in the presence of reduced nicotinamide adenine dinucleotide phosphate and oxygen to give three major identi(More)
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