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Genetic tool development in marine protists: emerging model organisms for experimental cell biology
TLDR
The development of genetic tools in a range of protists primarily from marine environments are reported on, providing a roadmap for developing genetically tractable organisms.
A reappraisal of the genus Leptocylindrus (Bacillariophyta), with the addition of three species and the erection of Tenuicylindrus gen. nov
TLDR
Despite the addition of the new taxa, the ancient diatom lineage of the Leptocylindraceae shows a relative species poorness and considerable morphological stasis.
Oxylipin Diversity in the Diatom Family Leptocylindraceae Reveals DHA Derivatives in Marine Diatoms
TLDR
The presence of species-specific lipoxygenase activity and oxylipin patterns is reported, providing the first demonstration of enzymatic production of docosahexaenoic acid derivatives in marine diatoms.
Assessment of Species Diversity and Distribution of an Ancient Diatom Lineage Using a DNA Metabarcoding Approach
TLDR
Despite the sequencing depth allowed by NGS and the wide geographic area covered by this study, the diversity of this ancient diatom family appears to be low, at least at the level of the marker used in this study.
Genetic tool development in marine protists: Emerging model organisms for experimental cell biology
TLDR
The reported breakthroughs on genetic manipulation position the community to dissect cellular mechanisms from a breadth of protists, which will collectively provide insights into ancestral eukaryotic lifeforms, protein diversification and evolution of cellular pathways.
Genetic, physiological and ecological diversity of the diatom genus Leptocylindrus
TLDR
A closer characterisation of Leptocylindrus species through different approaches, including the study of their morphology, molecular phylogeny, metagenomics and biochemistry is aimed at, to address the distribution of the 6 species outside the GoN.
Genome-enabled phylogenetic and functional reconstruction of an araphid pennate diatom Plagiostriata sp. CCMP470, previously assigned as a radial centric diatom, and its bacterial commensal
TLDR
Combined microscopic and molecular information is used to reclassify a diatom strain CCMP470, previously annotated as a radial centric species related to Leptocylindrus danicus, as an araphid pennate species in the staurosiroid lineage, within the genus Plagiostriata.
Diatom flagellar genes and their expression during sexual reproduction in Leptocylindrus danicus
TLDR
The diatom flagellar gene set appears to be more complete and functional than previously reported based on the genome sequence of the model centric diatom, Thalassiosira pseudonana, and paves the way to a wider recognition of the relevance of sexual reproduction in individual species and in the natural environment.
A ribosomal sequence-based oil sensitivity index for phytoplankton groups.
Aptamers are an innovative and promising tool for phytoplankton taxonomy and biodiversity research
TLDR
Aptamers discriminated also among cells of the same clone, suggesting a potential use of aptamers as clone-specific/stage-specific probes to track phytoplankton life stages in their natural environment.
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