Deepa Deshmukh

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Previously, it has been found that phenobarbital inhibited protein kinase C (PKC) and the enzymes of the metabolism of polyphosphoinositide, especially phosphatidylinositol 4-phosphate (PIP) kinase (PIP-kinase). As a continuation of these studies, a number of barbiturates (barbituric acid, barbital, butabarbital, pentobarbital, amobarbital, phenobarbital,(More)
Phosphomonoesterase and diesterase that cleave phosphatidylinositol-4-phosphate (diphosphoinositide, DPI) and phosphatidylinositol-4,5-bisphosphate (triphosphoinositide, TPI) were detected in three subfractions of purified rat brain myelin, and some properties of the enzymes were studied. Monoesterase activity was stimulated by KCl, maximally at a(More)
At intervals ranging from 1 to 10 min after injection of 32Pi into rat brain, myelin was prepared and separated into three subfractions: heavy, medium, and light. The radioactivity of total phospholipids and polyphosphoinositides (PPI) was then determined. There was rapid incorporation of 32Pi into PPI, which contained 50-70% of the radioactivity among(More)
Oligodendroglia were isolated from bovine brain, and a "crude" microsomal fraction obtained from cell homogenates was subfractionated into myelin (MP), plasma membranes (PM), Golgi (GF), smooth (SER) and rough (RER) endoplasmic membranes using discontinuous-sucrose gradient centrifugation. The submicrosomal fractions were characterized by ultrastructural(More)
Stroke is a leading cause of death for women, and midlife, low-income women are unlikely to appreciate their risk for stroke. The Well-Integrated Screening and Evaluation for Women Across the Nation program, administered by the Centers for Disease Control and Prevention Division for Heart Disease and Stroke Prevention, was developed for this population. The(More)
We describe an attempt to incorporate a metabolically inert phospholipid analog into animal membranes, especially myelin, in vivo, with the view of eventual long-term membrane modification or membrane engineering. A sonicated suspension of a mixture of [14C]phosphatidylcholine and its dialkyl analog, [3H] tetradecyloctadecano(1)phosphocholine, was injected(More)
Myelin basic protein and phosphatidylinositol-4-phosphate are phosphorylated in vitro by ATP and solubilized rat brain myelin. When both substrates are present together, the rate of phosphorylation of each is increased about eight-fold. It appears likely that the phosphate turnover of myelin basic protein and of phosphatidylinositol-4-phosphate are coupled(More)
— In the dissertation, structural Finite Element Analysis is carried out. Both linear and non linear analysis is considered for the investigation. Geometry non linearity, material non linearity and boundary non linearity also called as contact analysis is considered. All the boundary conditions, loadings, material definitions considered are real in nature.(More)
Sonicated emulsions of egg phosphatidylcholine containing either [(14)C]-dipalmitoyl phosphatidylcholine (diester-PC) or two metabolically inert analogs. [ (14)C]-1- octadecyl -2- hexadecyl -sn- glycero -3- phosphocholine (diether-PC) and [(3)H]-2-tetradecyloctadecano-(1)-phosphocholine (dialkyl-PC) were injected into the vitreous of the eye of adult(More)
When unilamellar "stable" liposomes composed of a dialkyl analog of phosphatidylcholine, tetradecyloctadec-11-eno(1)phosphocholine (dialkyl-PC), plus cholesterol at 1:1 molar ratio, and a trace of [3H]dialkyl-PC were injected into the vitreous of the rabbit eye, macrophage infiltration and phagocytosis of lipid were observed in retina including the(More)