Debprasad Patra

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Characterization of a mutant T7 RNA polymerase (RNAP) that is active on non-promoter templates but has lost the ability to selectively utilize the T7 promoter led to the finding that wild-type T7 RNAP initiates transcription at a high rate on non-promoter templates but that most (approximately 90%) of these initiation events lead to synthesis of(More)
We have isolated and characterized a number of bacteriophage T7 RNAP (RNA polymerase) null mutants. Most of the mutants found to be completely inactive in vitro map to one of the well-conserved blocks of residues in the family of RNAPs homologous to T7 RNAP. The in vitro phenotypes of a smaller number of partially active T7 RNAP mutants, mapping outside(More)
In order to test the proposal that most nucleotide polymerases share a common active site structure and folding topology, we have generated 22 mutations of residues within motifs A, B and C of T7 RNA polymerase (RNAP). Characterization of these T7 RNAP mutants showed the following: (i) most of the mutations resulted in moderate to drastic reductions in T7(More)
Ubiquitin-dependent degradation of intracellular proteins underlies a multitude of biological processes, including the cell cycle, cell differentiation, and responses to stress. One ubiquitin-dependent proteolytic system is the N-end rule pathway, whose targets include proteins that bear destabilizing N-terminal residues. This pathway, which has been(More)
The free ligands, 2-{(o-hydroxyaryl)azo}-1-N-salicylidene phenylamine, H(2)L [where H(2)L = RC(6)H(4)N=NC(6)H(4)N=CH-C(6)H(4)OHR = p-H for H(2)L(1), p-Me for H(2)L(2) and p-Cl for H(2)L(3)], were prepared by the condensation of salicylaldehyde with 2-{(o-hydroxy aryl)azo} aniline. Reaction of H(2)L with VOSO(4) afforded the oxovanadium complex,(More)
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