Deborah Wancio

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Early cortical progenitor cells of the ventricular zone (VZ) differ from later progenitor cells of the subventricular zone (SVZ) in cell-type generation and their level of epidermal growth factor receptors (EGFRs). To determine whether differences in their behavior are causally related to EGFR number/density, we introduced extra EGFRs into VZ cells with a(More)
Epidermal growth factor receptors (EGFRs) have been implicated in the control of migration in the telencephalon, but the mechanism underlying their contribution is unclear. We show that expression of a threshold level of EGFRs confers chemotactic competence in stem cells, neurons and astrocytes in cortical explants. This level of receptor expression is(More)
Previous studies demonstrated that the level of epidermal growth factor receptors (EGF-Rs) expressed by progenitor cells in the newborn (P0) rat retina was limiting for the generation of Muller glial cells but not for proliferation. To determine whether EGF-R signaling biases cells to generate a specific cell type or regulates more general processes during(More)
Sirolimus (rapamycin), a potent immunosuppressant in Phase II clinical trials, interrupts the immune response by interfering with mitogenic signal transduction following engagement of cytokine receptors [1]. The antiproliferative effects of this drug have been observed in a variety of cell types, and have been associated with inhibition of the activity of(More)
Fifty nanograms each of hamster antibodies to mouse CD3e and CD28 (Pharmingen, San Diego, CA) were fixed to 96 well round bottom plates coated with goat anti-hamster IgG (Jackson Immuno Research, West Grove, PA). T cells from the spleens of Balb/c mice were enriched by negative selection using column chromatography (R&D Systems, Minneapolis, MN) and plated(More)
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