Deborah Taylor

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Endometrial stromal cell culture Endometrial biopsies were obtained by curettage from consenting women aged 18-40 in accordance with local ethics committee requirements. Endometrial stromal cells were isolated and cultured as described previously (1). All experiments were performed within the first three cell passages. To induce decidualization in vitro,(More)
The use of cryopreserved human embryos in gene expression studies provides an additional source to the scarce embryos available for research. To validate their use we have implemented a quantitative RT-PCR to characterize the levels of the tuberous sclerosis, TSC2 gene in fresh and frozen-thawed human embryos. Frozen embryos were thawed using two different(More)
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