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Atmospheric concentrations of elemental mercury (Hg(0)), reactive gaseous Hg (RGM), and particulate Hg (pHg) concentrations were measured in Yellowstone National Park (YNP), U.S.A. using high resolution, real time atmospheric mercury analyzers (Tekran 2537A, 1130, and 1135). A survey of Hg(0) concentrations at various locations within YNP showed that(More)
A variety of factors have been investigated with regard to the quantitation of chemical species within individual ambient aerosol particles analyzed by laser desorption time-of-flight mass spectrometry. Spectrum to spectrum differences in the interaction of the particle with the ionization laser beam, which affect the absolute peak areas in the mass(More)
The distribution of gonadotropin-releasing hormone (GnRH) was studied immunohistochemically in the brain of the adult mouse with the peroxidase-antiperoxidase (PAP) method. Primary antisera were prepared against unconjugated synthetic GnRH and GnRH conjugated to limpet hemocyanin or bovine serum albumin. GnRH was localized in the organum vasculosum of the(More)
  • D S Gross
  • 1984
Despite its occurrence in most vertebrate species, the function of the hypophysial pars tuberalis (PT) remains obscure. Recent immunocytochemical studies have demonstrated the presence of hormone-containing cells in the few species studied. In the present study the secretory cell composition of the PT was characterized in a variety of mammals using(More)
Proton transfer reactivity of isolated charge states of the protein hen egg-white lysozyme shows that multiple distinct conformations of this protein are stable in the gas phase. The reactivities of the 9+ and 10+ charge state ions, formed by electrospray ionization of "native" (disulfide-intact) and "denatured" (disulfide-reduced) solutions, are consistent(More)
A relatively simple model for calculation of the energetics of gas-phase proton transfer reactions and the maximum charge state of multiply protonated ions formed by electrospray ionization is presented. This model is based on estimates of the intrinsic proton transfer reactivity of sites of protonation and point charge Coulomb interactions. From this(More)
The pars tuberalis and pars distalis are constant subdivisions of the vertebrate adenohypophysis. Unlike the pars distalis, however, direct evidence of an endocrine function for the pars tuberalis is lacking. The present immunocytochemical study shows the ovine pars tuberalis to be unique in that 1) its only immunoreactive hormone-containing cell type is(More)
Luteinizing hormone and follicle-stimulating hormone were identified by immunocytochemistry in the same cells of the pituitary pars tuberalis, following hypophysectomy in the rat. A marked hyperplasia and hypertrophy of these cells occurred in response to hypophysectomy. These findings are discussed in light of the potential for hormone production by pars(More)
The response of LH cells in the pars tuberalis of the rat hypophysis to castration alone or castration and subsequent administration of testosterone propionate or estradiol benzoate was studied. To accomplish this, LH cells were labeled by the peroxidase-antiperoxidase immunocytochemical method, with use of an antiserum generated against hCG. LH cells were(More)
The temperature dependence of the unimolecular kinetics for dissociation of the heme group from holo-myoglobin (Mb) and holo-hemoglobin alpha-chain (Hb-alpha) was investigated with blackbody infrared radiative dissociation (BIRD). The rate constant for dissociation of the 9 + charge state of Mb formed by electrospray ionization from a "pseudo-native"(More)