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  • D S Gross
  • 1984
Despite its occurrence in most vertebrate species, the function of the hypophysial pars tuberalis (PT) remains obscure. Recent immunocytochemical studies have demonstrated the presence of hormone-containing cells in the few species studied. In the present study the secretory cell composition of the PT was characterized in a variety of mammals using(More)
The response of LH cells in the pars tuberalis of the rat hypophysis to castration alone or castration and subsequent administration of testosterone propionate or estradiol benzoate was studied. To accomplish this, LH cells were labeled by the peroxidase-antiperoxidase immunocytochemical method, with use of an antiserum generated against hCG. LH cells were(More)
Since differential chemical staining has been unsatisfactory for demonstration of specific secretory cell types in the hypophyseal pars distalis of the mouse, the objective of this study was to determine whether the peroxidase-antiperoxidase immunocytochemical procedure might be more effective. Accordingly, representative sections from the hypophyses of 17(More)
Atmospheric concentrations of elemental mercury (Hg(0)), reactive gaseous Hg (RGM), and particulate Hg (pHg) concentrations were measured in Yellowstone National Park (YNP), U.S.A. using high resolution, real time atmospheric mercury analyzers (Tekran 2537A, 1130, and 1135). A survey of Hg(0) concentrations at various locations within YNP showed that(More)
The pars tuberalis and pars distalis are constant subdivisions of the vertebrate adenohypophysis. Unlike the pars distalis, however, direct evidence of an endocrine function for the pars tuberalis is lacking. The present immunocytochemical study shows the ovine pars tuberalis to be unique in that 1) its only immunoreactive hormone-containing cell type is(More)
The distribution of gonadotropin-releasing hormone (GnRH) was studied immunohistochemically in the brain of the adult mouse with the peroxidase-antiperoxidase (PAP) method. Primary antisera were prepared against unconjugated synthetic GnRH and GnRH conjugated to limpet hemocyanin or bovine serum albumin. GnRH was localized in the organum vasculosum of the(More)
Considerable evidence indicates that the pituitary-gonadal axis is active in the fetus, but little is known about the relative times in development when the brain begins to secrete gonadotropin-releasing hormone (GnRH) and the hypophysis begins to secrete gonadotropin. The objective was to correlate the time of appearance of GnRH in the median eminence and(More)
A relatively simple model for calculation of the energetics of gas-phase proton transfer reactions and the maximum charge state of multiply protonated ions formed by electrospray ionization is presented. This model is based on estimates of the intrinsic proton transfer reactivity of sites of protonation and point charge Coulomb interactions. From this(More)
A variety of factors have been investigated with regard to the quantitation of chemical species within individual ambient aerosol particles analyzed by laser desorption time-of-flight mass spectrometry. Spectrum to spectrum differences in the interaction of the particle with the ionization laser beam, which affect the absolute peak areas in the mass(More)
The endocrine function of the hypophysial pars tuberalis is currently unknown. Recent immunocytochemical and electron microscopic studies have shown the presence of gonadotropin-producing cells in this tissue in intact rats. Because the pars tuberalis is not destroyed by hypophysectomy, the objective of the present study was to determine whether this gland(More)