Deborah B. Henken

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In situ hybridization histochemistry was used to identify neurons in rat dorsal root ganglia that contained mRNAs encoding beta-preprotachykinin and preprosomatostatin. The distribution of these neurons was compared with the distribution of neurons containing tachykinins or somatostatin, identified using immunocytochemical techniques. Neurons labelled for(More)
In a previous study we described two distinct neuronal phenotypes in rat dorsal root ganglia based on immunocytochemical assays for the neuronal intermediate filament proteins, peripherin and low-molecular-weight neurofilaments [Goldstein M. E. et al. (1991) J. Neurosci. Res. 30, 92-104]. In this paper we have extended this classification by using in situ(More)
In animal models, spread of herpes simplex virus type 1 (HSV-1) from epithelial replication sites to the peripheral and central nervous system is known from analysis of individually dissected tissues. To examine virus spread in undissociated tissues, corneas of adult mice were inoculated with HSV-1. After 1 to 13 days groups of mice were perfused with(More)
Cumulative evidence suggests that varicella-zoster virus (VZV) can infect walls of CNS arteries, causing stroke in man. We review observations relating infection with this neurotropic virus to the development of arteritis in the CNS and note evidence supporting the hypothesis that VZV spreads from ganglionic reactivation sites to the arterial wall by neural(More)
We examined the effects of herpes simplex virus type 2 (HSV-2) infection on host neuropeptide content in mouse dorsal root ganglia (DRG) neurons following unilateral hind footpad inoculation. At selected survival times following infection, adjacent tissue sections of decalcified spine containing the paired 4th and 5th lumbar DRGs were immunoreacted to(More)
To investigate whether herpes simplex virus type-2 (HSV-2) infection of sensory ganglion neurons is restricted to a particular neuronal class, we unilaterally inoculated either the footpad, leg muscle or sciatic nerve of mice with strains of HSV-2 that differ in virulence and examined the soma sizes of lumbar dorsal root ganglion (DRG) neurons containing(More)
The changes in gene expression and protein synthesis induced in neurons by axotomy usually lead to increased production of axon constituents and decreased production of molecules related to neurotransmission. Exceptions to this generalization occur, however, and it is unclear whether the injury itself changes the pattern of synthesis or whether individual(More)
The National Institutes of Health (NIH) has been a leading advocate of the zebrafish as a model organism for the study of vertebrate development, physiology, and disease. Genomic tools, developed with the support of NIH funding, have made zebrafish even more attractive as a genetic system and have stimulated research using this model. The NIH continues to(More)
We examined whether EDTA decalcification decreases the sensitivity of antigen detection of formalin-fixed paraffin-embedded neural tissues. In undissected decalcified tissues, the immunoreactivities of select neural antigens were quantitatively compared with those in dissected sensory ganglia. Decalcification does not reduce either the numbers or staining(More)
The National Institutes of Health (NIH), an agency in the Department of Health and Human Services (DHHS), is a strong advocate of zebrafish and other animal model systems for biomedical and behavior research. In part because of strong funding support from NIH, zebrafish research is now providing fundamental insights into physiology, behavior, and the(More)