Deborah B Henken

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In a previous study we described two distinct neuronal phenotypes in rat dorsal root ganglia based on immunocytochemical assays for the neuronal intermediate filament proteins, peripherin and low-molecular-weight neurofilaments [Goldstein M. E. et al. (1991) J. Neurosci. Res. 30, 92-104]. In this paper we have extended this classification by using in situ(More)
The National Institutes of Health (NIH), an agency in the Department of Health and Human Services (DHHS), is a strong advocate of zebrafish and other animal model systems for biomedical and behavior research. In part because of strong funding support from NIH, zebrafish research is now providing fundamental insights into physiology, behavior, and the(More)
The National Institutes of Health (NIH) has been a leading advocate of the zebrafish as a model organism for the study of vertebrate development, physiology, and disease. Genomic tools, developed with the support of NIH funding, have made zebrafish even more attractive as a genetic system and have stimulated research using this model. The NIH continues to(More)
In situ hybridization histochemistry was used to identify neurons in rat dorsal root ganglia that contained mRNAs encoding beta-preprotachykinin and preprosomatostatin. The distribution of these neurons was compared with the distribution of neurons containing tachykinins or somatostatin, identified using immunocytochemical techniques. Neurons labelled for(More)
Cumulative evidence suggests that varicella-zoster virus (VZV) can infect walls of CNS arteries, causing stroke in man. We review observations relating infection with this neurotropic virus to the development of arteritis in the CNS and note evidence supporting the hypothesis that VZV spreads from ganglionic reactivation sites to the arterial wall by neural(More)
We have examined the effects of herpes simplex virus type 2 (HSV-2) infection on neuron numbers in mouse dorsal root ganglia (DRG) following unilateral hind footpad inoculation. One month following HSV-2 strain MS inoculation, tissue sections of decalcified spine containing the paired 4th and 5th lumbar DRGs were stained with cresyl violet. Neuronal(More)
We examined whether EDTA decalcification decreases the sensitivity of antigen detection of formalin-fixed paraffin-embedded neural tissues. In undissected decalcified tissues, the immunoreactivities of select neural antigens were quantitatively compared with those in dissected sensory ganglia. Decalcification does not reduce either the numbers or staining(More)
In animal models, spread of herpes simplex virus type 1 (HSV-1) from epithelial replication sites to the peripheral and central nervous system is known from analysis of individually dissected tissues. To examine virus spread in undissociated tissues, corneas of adult mice were inoculated with HSV-1. After 1 to 13 days groups of mice were perfused with(More)
We examined the effects of herpes simplex virus type 2 (HSV-2) infection on host neuropeptide content in mouse dorsal root ganglia (DRG) neurons following unilateral hind footpad inoculation. At selected survival times following infection, adjacent tissue sections of decalcified spine containing the paired 4th and 5th lumbar DRGs were immunoreacted to(More)
This research examines changes that occur in neurons during corneal herpes simplex virus (HSV-1) infection and focuses on the nerve growth associated protein GAP-43. Cornea and trigeminal ganglion (TG) of New Zealand white rabbits were examined after inoculation of the McKrae and 17 Syn+ strains of HSV-1 to the cornea. Rabbit tissues were taken during(More)