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Transient forebrain ischemia induces delayed, selective neuronal death in the CA1 region of the hippocampus. The underlying molecular mechanisms are as yet unclear, but it is known that activation of L-type Ca2+ channels specifically increases the expression of a group of genes required for neuronal survival. Accordingly, we examined temporal changes in(More)
Activation of N-methyl-d-aspartic acid (NMDA) receptor plays an important role in neuronal apoptosis induced by cerebral ischemia but the underlying mechanisms are still unclear. The present study examined the neuroprotection of three chloride blockers in an in vitro cell model of cerebral ischemia established by treatment of cultured rat hippocampal(More)
OBJECTIVE To study the localization of L-type calcium channel alpha1 subunits CaV1.2alpha1C and CaV1.3alpha1D in CA1 and CA3 regions of adult rat hippocampus. METHODS Immunohistochemical staining was employed for specific labeling of alpha1 subunits CaV1.2alpha1C and CaV1.3alpha1D. RESULTS CaV1.2alpha1C subunit was mainly located in the apical and basal(More)
OBJECTIVE To investigate the effect of liquid extract of Lishi No.5 formula in protecting the neurons from excitatory amino acid injury. METHODS Cultured neonatal SD rat hippocampal neurons were treated with 300 micromol/L NMDA and 5 micromol/L glycine for 10 min, and the conditioned culture medium was replaced by normal culture medium. After cell culture(More)
OBJECTIVE To investigate changes in synaptic and extrasynaptic N-methyl-D-aspartate receptors (NMDAR) during the development of cultured rat hippocampal neurons. METHODS Synaptic and extrasynaptic NMDAR channel currents were recorded from 1-day-old rat hippocampal neurons cultured for 1 and 2 weeks with patch-clamp technique in whole-cell configuration(More)
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