Dayna K. Mudge

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While the counterselectable Schizosaccharomyces pombe ura4 + gene can be used to prepare a site in the S. pombe genome to receive an unmarked mutant allele (loss of ura4 + confers 5FOA-resistant (5FOAR) growth), the desired unmarked knock-in strains are generally outnumbered by spontaneously arising 5FOAR mutants. Relative to the same approach using the(More)
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