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We evaluated West Nile virus (WNV) seroprevalence in dogs and cats in Shanghai, China. Seventeen of the 367 dogs (4.6%) and 46 of the 309 cats (14.9%) tested positive for WNV antibodies. A higher WNV seroprevalence was found with outdoor and rural pets than with indoor and urban pets. However, WNV seroprevalence between the sexes were not significantly(More)
The present study describes the development and validation of a one-step, single-tube, and real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) detecting Porcine Sapelovirus. RT-LAMP characterized by one strand displacement reaction with the specific stem-loop structure and Bst DNA polymerase could be finished in 60 min under(More)
BACKGROUND Hepatitis E virus (HEV) is a major causative agent of acute clinical hepatitis in adults through much of Asia, the Middle East and Africa. Open reading frame 3 (ORF3) encodes around 120 amino acids of phosphorylation protein that associates with the cytoskeleton, while its precise biological function is still unknown. OBJECTIVES In order to(More)
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