Image sensors capable of resolving the time-of-arrival (ToA) of individual pho-tons with high resolution are needed in several applications, such as fluores-cence lifetime imaging microscopy (FLIM), Förster resonance energy transfer (FRET), optical rangefinding, and positron emission tomography. In FRET, for example, typical fluorescence lifetime is of the… (More)
We have successfully demonstrated video-rate CMOS single-photon avalanche diode (SPAD)-based cameras for fluorescence lifetime imaging microscopy (FLIM) by applying innovative FLIM algorithms. We also review and compare several time-domain techniques and solid-state FLIM systems, and adapt the proposed algorithms for massive CMOS SPAD-based arrays and… (More)
Table 1 of an earlier paper [Opt. Lett.40, 336 (2015)10.1364/OL.40.000336] contained an incorrect mathematical expression. The error is rectified here.
Estimation of signal parameters via rotational invariance techniques is a classical algorithm widely used in array signal processing for direction-of-arrival estimation of emitters. Inspired by this method, a new signal model and new fluorescence lifetime estimation via rotational invariance techniques (FLERIT) were developed for multiexponential… (More)
Fluorescence lifetime of dye molecules is a sensitive reporter on local microenvironment which is generally independent of fluorophores concentration and can be used as a means of discrimination between molecules with spectrally overlapping emission. It is therefore a potentially powerful multiplexed detection modality in biosensing but requires extremely… (More)