The DDB1-CUL4ADDB2 ubiquitin ligase is deficient in xeroderma pigmentosum group E and targets histone H2A at UV-damaged DNA sites.
- M. Kapetanaki, Jennifer Guerrero-Santoro, Dawn C. Bisi, C. Hsieh, V. Rapić-Otrin, A. Levine
- BiologyProceedings of the National Academy of Sciences…
- 21 February 2006
It is demonstrated that monoubiquitinated histone H2A in native chromatin coimmunoprecipitates with the endogenous DDB1-CUL4A(DDB2) complex in response to UV irradiation, suggesting that histone modification, mediated by the XPE factor, facilitates the initiation of NER.
Sequential binding of UV DNA damage binding factor and degradation of the p48 subunit as early events after UV irradiation.
- V. Rapić-Otrin, M. McLenigan, Dawn C. Bisi, Martín Gonzalez, A. Levine
- BiologyNucleic Acids Research
- 1 June 2002
It is reported here that an early event after UV, but not ionizing, radiation is the transient dose-dependent degradation of the small subunit, p48, and a functional connection between UV-DDB binding activity, proteasomal degradation of p48 and chromatin remodeling during early steps of NER is supported.
True XP group E patients have a defective UV-damaged DNA binding protein complex and mutations in DDB2 which reveal the functional domains of its p48 product.
- V. Rapić-Otrin, Valentina Navazza, M. Stefanini
- BiologyHuman Molecular Genetics
- 1 July 2003
It is confirmed that the true XP-E phenotype is DDB(-), resulting from defects in a single gene, DDB2, and the functional domains of the corresponding p48 protein are identified.
Secretion of Anti-Plasmodium Effector Proteins from a Natural Pantoea agglomerans Isolate by Using PelB and HlyA Secretion Signals
- Dawn C. Bisi, D. Lampe
- BiologyApplied and Environmental Microbiology
- 20 May 2011
P. agglomerans, a bacterial symbiont of Anopheles mosquitoes, could be engineered to express and secrete anti-Plasmodium effector proteins, a strategy termed paratransgenesis that may be expected to be competitive with the native microbiota in the environment of the mosquito midgut.
A modifier screen identifies regulators of cytoskeletal architecture as mediators of Shroom-dependent changes in tissue morphology
- J. Hildebrand, Adam D Leventry, N. Kaufmann
- BiologyBiology Open
- 27 January 2021
Using Drosophila as a model system, F-actin and microtubules are identified as important determinants of how cells and tissues respond to Shroom induced contractility.
Engineering the Mosquito Symbiont Pantoea agglomerans to secrete Anti-Plasmodium Inhibitory Proteins
- Dawn C. Bisi
- Biology, Medicine
- 2009
It was hypothesized that the coding sequence of a native secreted protein could serve as the secretion signal and the best candidate was FliC/Flagellin, which is the major component of the bacterial flagellum and is known to be secreted from the cell in a Type III secretion system-like manner.