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OBJECTIVE To investigate apoptosis of bovine trabecular meshwork cells induced by dexamethasone. METHODS In the incubation method of system in vitro, dexamethasone (1 - 500 mg/L) was added into the syncretic culture solution of the third to fifth generation of bovine trabecular meshwork cells. After 1 - 14 days, the culture solution was observed by(More)
PURPOSE To study the molecular mechanism of trabecular meshwork cells apoptosis induced by dexamethasone and the protection of pilocarpine. METHODS Determining mRNA expression with reverse transcription-polymerase chain protein expression with Western blots and the percentage of reaction (RT-PCR), apoptotic cells with fluorescent microscopy. RESULTS(More)
As a functionally unique subset of T cells, regulatory T cells (Treg) suppress tumor immune responses effectively through a variety of mechanisms and play an important role in tumorigenesis and tumor progression. There is growing evidence to suggest that Treg participates in the formation and development of hepatic tumor, especially the HCC. Elucidation of(More)
This paper deals with the ultrastructure of the body wall of adult Pagumogonimus skrjabini by transmission electron microscopy. Infected crabs were collected from Siyen, Hubei Province, and adult worms were obtained from the lungs of experimentally infected dogs 90 days post-infection. The normal structure of body wall of the P. skrjabini is composed of(More)
OBJECTIVE To explore the effect of calcium ionophore (CI) A23187 plus IFN-γ on dendritic cells (DC) from healthy human peripheral blood mononuclear cells (PBMNC). METHODS PBMNC from healthy donors were treated with GM-CSF plus IL-4, A23187, and A23187 plus IFN-γ, respectively. After culture for 72 h, the change of cellular morphology was observed under(More)
PURPOSE To determine whether the temperature-sensitive rat retinal pigment epithelial cell line (RPE-J) express mRNA transcripts for apolipoprotein(apo) and microsomal triglyceride transfer protein (MTP) and whether it can synthesize and secrete neutral lipids. METHODS RPE-J cells were cultured in plastic tissue culture flasks and on Transwell filters.(More)
PURPOSE To study whether muscarinic receptor agonists can protect cultured bovine trabecular meshwork cells against apoptosis induced by dexamethasone. METHODS The third to fifth passages of bovine trabecular meshwork cells were grown to confluence and incubated for 1-14 days in growth media with dexamethasone or pretreatment of pilocarpine or carbachol.(More)
We aimed to assess parameters reflecting left ventricular function by dual-source computed tomography (DSCT) with echocardiography (ECG) as control. Fifty-eight patients with coronary heart disease (CHD) were recruited from January to June 2011; 29 CHD patients had type II diabetes. All patients were assessed by cardiac DSCT and ECG examination. DSCT and(More)
The spermiogenesis of Pagumogonimus skrjabini was observed on the ultrastructural level by transmission electron microscopy. The development and the morphological characteristics of spermatids and sperm were studied. The Golgi comples was located near the anterior region of the spermatid nucleus and developed into an acrosomal cap of the sperm. Accordingly,(More)