David Svec

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The interest to analyze single and few cell samples is rapidly increasing. Numerous extraction protocols to purify nucleic acids are available, but most of them compromise severely on yield to remove contaminants and are therefore not suitable for the analysis of samples containing small numbers of transcripts only. Here, we evaluate 17 direct cell lysis(More)
We have examined the imprecision in the estimation of PCR efficiency by means of standard curves based on strategic experimental design with large number of technical replicates. In particular, how robust this estimation is in terms of a commonly varying factors: the instrument used, the number of technical replicates performed and the effect of the volume(More)
Genomic DNA (gDNA) contamination is an inherent problem during RNA purification that can lead to non-specific amplification and aberrant results in reverse transcription quantitative PCR (RT-qPCR). Currently, there is no alternative to RT(-) controls to evaluate the impact of the gDNA background on RT-PCR data. We propose a novel method (ValidPrime) that is(More)
OBJECTIVE Quantification of small molecule numbers often requires preamplification to generate enough copies for accurate downstream enumerations. Here, we studied experimental parameters in targeted preamplification and their effects on downstream quantitative real-time PCR (qPCR). METHODS To evaluate different strategies, we monitored the(More)
  • Anthony G Oettinger, John C B Legates, Joseph Butcher, David Sulek, Erin Macdougall, Katie Hines +37 others
  • 2002
All of the authors work at Booz Allen Hamilton. David Sulek (Principal Investigator) is a Senior Associate specializing in analysis of national security, homeland security, and public-private partnership policy issues. Joseph Butcher (Principal Investigator) is an Associate specializing in telecommunications and Internet policy and security analysis. Erin(More)
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