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Double-slit electron interferometers fabricated in high mobility two-dimensional electron gases are powerful tools for studying coherent wave-like phenomena in mesoscopic systems. However, they suffer from low visibility of the interference patterns due to the many channels present in each slit, and from poor sensitivity to small currents due to their open(More)
The Notch-Delta signalling pathway allows communication between neighbouring cells during development. It has a critical role in the formation of 'fine-grained' patterns, generating distinct cell fates among groups of initially equivalent neighbouring cells and sharply delineating neighbouring regions in developing tissues. The Delta ligand has been shown(More)
The complex genetic circuits found in cells are ordinarily studied by analysis of genetic and biochemical perturbations. The inherent modularity of biological components like genes and proteins enables a complementary approach: one can construct and analyse synthetic genetic circuits based on their natural counterparts. Such synthetic circuits can be used(More)
Developmental patterning requires juxtacrine signaling in order to tightly coordinate the fates of neighboring cells. Recent work has shown that Notch and Delta, the canonical metazoan juxtacrine signaling receptor and ligand, mutually inactivate each other in the same cell. This cis-interaction generates mutually exclusive sending and receiving states in(More)
We measured the phase evolution of electrons as they traverse a quantum dot (QD) formed in a two-dimensional electron gas that serves as a localized spin. The traversal phase, determined by embedding the QD in a double path electron interferometer and measuring the quantum interference of the electron wave functions manifested by conductance oscillation as(More)
The settling time of the droplets depends on the viscosity of the silicate melt. For the experiment of long duration at high temperature, the amount of H 2 O in the starting material should be reduced to make the settling time longer to prevent interaction between the droplets and the capsule wall. In the experiments with various amounts of H 2 O in the(More)
The Notch signaling pathway consists of multiple types of receptors and ligands, whose interactions can be tuned by Fringe glycosyltransferases. A major challenge is to determine how these components control the specificity and directionality of Notch signaling in developmental contexts. Here, we analyzed same-cell (cis) Notch-ligand interactions for(More)
Construct Name Promoter Gene Mammalian Selection Role in this work pEV‐UAS‐H2B‐citrine UAS H2B‐citrine Zeocin Reporter for hN1‐Gal4 esn pEV‐12xCSL‐H2B‐ citrine 12xCSL H2B‐citrine Zeocin Reporter for hN1 pcDNA3‐hN1‐mod1 CMV hNotch1 Neomycin hN1 construct pcDNA3‐hN1‐ mcherry CMV hN1‐mcherry Neomycin hN1 construct (used) pCDNA3‐hNECD – Gal4 esn CMV hNECD –(More)
Lateral inhibition patterns mediated by the Notch-Delta signaling system occur in diverse developmental contexts. These systems are based on an intercellular feedback loop in which Notch activation leads to down-regulation of Delta. However, even in relatively well-characterized systems, the pathway leading from Notch activation to Delta repression often(More)
Theoretical and computational approaches for understanding different aspects of Notch signaling and Notch dependent patterning are gaining popularity in recent years. These in silico methodologies can provide dynamic insights that are often not intuitive and may help guide experiments aimed at elucidating these processes. This chapter is an introductory(More)