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The Notch-Delta signalling pathway allows communication between neighbouring cells during development. It has a critical role in the formation of 'fine-grained' patterns, generating distinct cell fates among groups of initially equivalent neighbouring cells and sharply delineating neighbouring regions in developing tissues. The Delta ligand has been shown(More)
Developmental patterning requires juxtacrine signaling in order to tightly coordinate the fates of neighboring cells. Recent work has shown that Notch and Delta, the canonical metazoan juxtacrine signaling receptor and ligand, mutually inactivate each other in the same cell. This cis-interaction generates mutually exclusive sending and receiving states in(More)
The complex genetic circuits found in cells are ordinarily studied by analysis of genetic and biochemical perturbations. The inherent modularity of biological components like genes and proteins enables a complementary approach: one can construct and analyse synthetic genetic circuits based on their natural counterparts. Such synthetic circuits can be used(More)
A capability for scanning electron microscopy of wet biological specimens is presented. A membrane that is transparent to electrons protects the fully hydrated sample from the vacuum. The result is a hybrid technique combining the ease of use and ability to see into cells of optical microscopy with the higher resolution of electron microscopy. The(More)
The Notch signaling pathway consists of multiple types of receptors and ligands, whose interactions can be tuned by Fringe glycosyltransferases. A major challenge is to determine how these components control the specificity and directionality of Notch signaling in developmental contexts. Here, we analyzed same-cell (cis) Notch-ligand interactions for(More)
Construct Name Promoter Gene Mammalian Selection Role in this work pEV‐UAS‐H2B‐citrine UAS H2B‐citrine Zeocin Reporter for hN1‐Gal4 esn pEV‐12xCSL‐H2B‐ citrine 12xCSL H2B‐citrine Zeocin Reporter for hN1 pcDNA3‐hN1‐mod1 CMV hNotch1 Neomycin hN1 construct pcDNA3‐hN1‐ mcherry CMV hN1‐mcherry Neomycin hN1 construct (used) pCDNA3‐hNECD – Gal4 esn CMV hNECD –(More)
Theoretical and computational approaches for understanding different aspects of Notch signaling and Notch dependent patterning are gaining popularity in recent years. These in silico methodologies can provide dynamic insights that are often not intuitive and may help guide experiments aimed at elucidating these processes. This chapter is an introductory(More)
Lateral inhibition patterns mediated by the Notch-Delta signaling system occur in diverse developmental contexts. These systems are based on an intercellular feedback loop in which Notch activation leads to down-regulation of Delta. However, even in relatively well-characterized systems, the pathway leading from Notch activation to Delta repression often(More)
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