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The Schlafen (Slfn) family of genes are differentially regulated during thymocyte maturation and are preferentially expressed in the lymphoid tissues. Ectopic expression of the prototype member Slfn1 early in the T lineage profoundly alters cell growth and development. In these mice, the DP thymocytes fail to complete maturation, and, depending on the(More)
We have used a rat model of focal cerebral ischemia to investigate changes in gene expression that occur during stroke. To monitor these changes, we employed representational difference analysis-polymerase chain reaction (PCR). A total of 128 unique gene fragments were isolated, and we selected 13 of these for quantitative reverse transcriptase-PCR(More)
The ventral tegmental area (VTA) forms part of the mesocorticolimbic system and plays a pivotal role in reward and reinforcing actions of drugs of abuse. Glutamate transmission within the VTA controls important aspects of goal-directed behavior and motivation. Noradrenergic receptors also present in the VTA have important functions in the modulation of(More)
Protein folding refers to the process whereby a protein assumes its intricate three-dimensional shape. This chapter reviews a class of methods for studying the folding process called roadmap methods. The goal of these methods is not to predict the folded structure of a protein, but rather to analyze the folding kinetics. It is assumed that the folded state(More)
Different potencies for hypocretin-1 and -2 in sleep-wake regulation and feeding after intracerebroventricular (ICV) administration have been reported. These differences were often explained by the selectivity of the two hypocretins for hypocretin receptor-1 and -2, but little attention has been paid to kinetics of hypocretin peptides. We investigated the(More)
The CXC chemokine receptor 3 (CXCR3) is predominantly expressed on T helper type 1 (Th1) cells that are involved in inflammatory diseases. The three CXCR3 ligands CXCL9, CXCL10, and CXCL11 are produced at sites of inflammation and elicit migration of pathological Th1 cells. Here, we are the first to characterize the pharmacological potencies and specificity(More)
Personal robots will contribute mobile manipulation capabilities to our future smart homes. In this paper, we propose a low-cost object localization system that uses static devices with Bluetooth capabilities , which are distributed in an environment, to detect and localize active Bluetooth beacons and mobile devices. This system can be used by a robot to(More)
The activity of most drugs is regulated by the binding of one molecule (the lig-and) to a pocket of another, usually larger, molecule, which is commonly a protein. This report describes a new approach to creating low-energy structures of flexible proteins to which ligands can be docked. The flexibility of molecules is encoded with thousands of parameters(More)
Chronic cocaine administration leads to catecholamine reuptake inhibition which enhances reward and motivational behaviors. Ventral Tegmental Area dopaminergic (VTA DA) neuronal firing is associated with changes in reward predictive signals. Acute cocaine injections inhibit putative VTA DA cell firing in vertebrates. Parthenolide, a compound isolated from(More)