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The CRISPR-Cas9 system has the potential to revolutionize genome editing in human pluripotent stem cells (hPSCs), but its advantages and pitfalls are still poorly understood. We systematically tested the ability of CRISPR-Cas9 to mediate reporter gene knockin at 16 distinct genomic sites in hPSCs. We observed efficient gene targeting but found that targeted(More)
The rapid rate of change in technology and the increasing sophistication of cyber attacks require any organization to have a continuous preparation. However, the resource and time intensive nature of cybersecurity education and training renders traditional approaches highly inefficient. Simulators have attracted the attention in the last years as a(More)
Hepatic lipase (HL) and endothelial lipase (EL) are negative regulators of plasma HDL cholesterol (HDLc) levels and presumably could affect two main HDL atheroprotective functions, macrophage-to-feces reverse cholesterol transport (RCT) and HDL antioxidant properties. In this study, we assessed the effects of both HL and EL deficiency on macrophage-specific(More)
BACKGROUND Type 2 diabetes mellitus (T2D) is associated with higher cardiovascular risk partly related to an increase in inflammatory parameters. The aim of this study was to determine the association of inflammatory biomarkers with low-density lipoprotein (LDL) subfraction phenotype and glycemic control in subjects with T2D and poor glycemic control. (More)
In modern societies, due to the high crime rates and high traffic accidents the feeling of insecurity and threat is increasing. The need for the establishment of defence and prevention mechanisms has encouraged studies to develop automatic recognition systems, which should, for example, have the ability to recognize vehicles at a certain distance.
A real time room acoustics auralization system for arbitrarily shaped rooms is presented. The system is based on an accelerated image source method and a time-dependent radiosity method for the computation of the complete binaural room impulse response. The auralization module is implemented as a wide band frequency dependence system. Elements such as wall(More)
Promotion of reverse cholesterol transport (RCT) is thought to be a major HDL-mediated mechanism for protecting against atherosclerosis. Preclinical studies support the concept that increasing cholesterol efflux from macrophages may confer atheroprotective benefits independently of the plasma HDL-cholesterol concentration. The application of the(More)
SCOPE The present study aimed to compare the effects of diets containing high-fat, high-cholesterol and saturated fatty acids (HFHC-SFA) and HFHC-polyunsaturated fatty acids-containing (HFHC-PUFA) diets on two major antiatherogenic functions of HDL, the HDL antioxidant function and the macrophage-to-feces reverse cholesterol transport. METHODS AND RESULTS(More)
Genome editing of human pluripotent stem cells (hPSCs) with the CRISPR/Cas9 system has the potential to revolutionize hPSC-based disease modeling, drug screening, and transplantation therapy. Here, we aim to provide a single resource to enable groups, even those with limited experience with hPSC culture or the CRISPR/Cas9 system, to successfully perform(More)
Because of the diversity of portfolios based on assets throughout international markets, exchange rate prediction plays an important role in risk management, asset allocation, and trading strategies. This paper aims to investigate the use of a recent paradigm of recurrent neural networks, echo state networks (ESNs), applied to forecasting and trading(More)