David R. Rockhold

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Five cDNAs encoding a putative soluble epoxide hydrolase (sEH) from potato were isolated and characterized. The cDNAs contained open reading frames encoding 36 kDa polypeptides which were highly homologous to the carboxy terminal region of mammalian sEH. When one of the cDNAs was expressed in a baculovirus system a soluble 38 kDa protein with epoxide(More)
Transformed Russet Burbank and Lemhi Russet clones which contained three different transgene constructs were evaluated for performance under field conditions in Idaho. The transgenic lines were characterized over two growing seasons, using plants grown from both greenhouse produced minitubers (first year) and field grown seed (second year). Individual(More)
Binary plant transformation vectors are widely used for introduction of transgenes into plants via Agrobacterium tumefaciens-mediated transformation. We report the construction of a binary plant vector pBINPLUS/ARS based on the pBINPLUS vector. Improvements introduced into pBINPLUS/ARS include the use of nonproprietary (ubiquitin-3 gene of Solanum(More)
To provide tools for breeders to control the steroidal glycoalkaloid (SGA) pathway in potato, we have investigated the steroidal alkaloid glycosyltransferase (Sgt) gene family. The committed step in the SGA pathway is the glycosylation of solanidine by either UDP-glucose or UDP-galactose leading to alpha-chaconine or alpha-solanine, respectively. The Sgt2(More)
The stress-induced expression of four different ubiquitin-encoding cDNAs was characterized in potato tuber tissue. The four clones exhibited differences in both structure and expression. The first cDNA encoded a single ubiquitin unit fused to an 80 amino acid ribosomal extension protein identical to the extension protein from tomato. Accumulation of the(More)
Steroidal glycoalkaloids (SGAs) are potentially harmful specialty metabolites found in Solanaceous plants. Two tri-glycosylated alkaloids, alpha-chaconine and alpha-solanine accumulate in potato tubers. Expressed sequence tags (ESTs) were identified in the available database by searching for protein homology to the Sgt1 (SOLtu:Sgt1) steriodalalkaloid(More)
Steroidal glycoalkaloids (SGAs) are secondary metabolites of Solanaceous plants. Two predominant glycoalkaloids, a-chaconine and asolanine are produced in potatoes. An antisense transgene was constructed to down-regulate glycoalkaloid biosynthesis using a potato cDNA encoding a sterol alkaloid glycosyltransferase (Sgt1). Introduction of this construct into(More)
Phytophthora infestans, the causal agent of late blight is the most devastating pathogen of cultivated potato worldwide. Utilizing map based cloning; a genomic region containing a cluster of six nucleotide binding site-leucine-rich repeat resistance gene analogs was isolated from the wild potato species Solanum bulbocastanum. Four genes were pseudogenes,(More)
Contour-clamped homogeneous electric field (CHEF) gel electrophoresis was used to separate intact, chromosome-size DNA of different species of Saccharomyces and Zygosaccharomyces. Strains of the same Saccharomyces species had similar electrophoretic karyotypes. However, reproducible differences between individual bands indicated that strain-specific(More)
Two polyubiquitin genes, bul409 and bul427, were isolated from a Solanum bulbocastanum BAC library. The bul409 and bul427 genes encode hexameric and heptameric polyproteins, respectively. bul427 exhibits a number of features suggestive of a pseudogene: (1) The last ubiquitin monomer of bul427 is interrupted by a frame shift mutation. (2) The coding sequence(More)