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Analysis of a TnblaM mutant of Brucella suis 1330, identified as being unable to multiply in Hela cells, allowed us to identify a 11 860 bp region of the B. suis genome encoding a type IV secretion system, homologous to the VirB system of Agrobacterium tumefaciens and the Ptl system of Bordetella pertussis. DNA sequence revealed 12 open reading frames(More)
Computational Grids are distributed systems that provide access to computational resources in a transparent fashion. Collecting and providing information about the status of the Grid itself is called Grid monitoring. We describe R-GMA (Relational Grid Monitoring Architecture) as a solution to the Grid monitoring problem. It uses a local as view approach to(More)
We have developed and implemented the Relational Grid Monitoring Architecture (R-GMA) as part of the DataGrid project, to provide a flexible information and monitoring service for use by other middleware components and applications. R-GMA presents users with a virtual database and mediates queries posed at this database: users pose queries against a global(More)
A type IV secretion system similar to the VirB system of the phytopathogen Agrobacterium tumefaciens is essential for the intracellular survival and multiplication of the mammalian pathogen Brucella. Reverse transcriptase-PCR showed that the 12 genes encoding the Brucella suis VirB system form an operon. Semiquantitative measurements of virB mRNA levels by(More)
Brucella melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep and Malta fever in humans. The genome of B. melitensis strain 16M was sequenced and found to contain 3,294,935 bp distributed over two circular chromosomes of 2,117,144 bp and 1,177,787 bp encoding 3,197 ORFs. By using the bioinformatics suite ERGO,(More)
The effects of milk yield, body condition score (BCS) and lactation number on the number of oocytes recovered and blastocysts formed were studied following in vitro maturation, fertilization and culture of bovine oocytes collected from 48 high and 46 medium genetic merit dairy cows in their first and third lactation. The cows were slaughtered between 125(More)
Two experiments were carried out to study the effect of nutrition on embryo development in two periods in superovulated ewes (Expt 1) and on oocyte developmental capacity during the late follicular phase (Expt 2). In Expt 1, a lower superovulation response in terms of animals ovulating (P < 0.05), ovulation rate per ewe ovulating (P = 0.1) and number of(More)
Acid pH is an environmental stress often encountered by Brucella during both the "environmental" and the "pathogenic" stages of its life. We have investigated the behaviour of B. suis biovar 1 and B. canis in acid conditions. Growth at suboptimal pH was characterized by a dramatic reduction in growth yield due to an early onset of stationary phase. B. suis(More)
We describe R-GMA (Relational Grid Monitoring Architecture) which is being developed within the European DataGrid Project as an Grid Information and Monitoring System. Is is based on the GMA from GGF, which is a simple Consumer-Producer model. The special strength of this implementation comes from the power of the relational model. We offer a global view of(More)
PacI and SpeI restriction maps were obtained for the two chromosomes of each of the six species of the genus Brucella: B. melitensis, B. abortus, B. suis, B. canis, B. ovis, and B. neotomae. Three complementary techniques were used: hybridization with the two replicons as probes, cross-hybridization of restriction fragments, and a new mapping method. For(More)