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T4 contains two groups of genes with similarity to homing endonucleases, the seg-genes (similarity to endonucleases encoded by group I introns) containing GIY-YIG motifs and the mob-genes (similarity to mobile endonucleases) containing H-N-H motifs. The four seg-genes characterized to date encode homing endonucleases with cleavage sites close to their(More)
37 (85%) von 44 menschlichen Mammacarcinomen konnten erfolgreich ohne zusätzlich immunsuppressive Therapie auf thymusaplastische nu/nu-Mäuse transplantiert werden. 16 Wochen nach der Transplantation zeigten 4 relativ schnell wachsende Tumoren einen menschlichen, weiblichen Chromosomensatz. Bei folgenden Untersuchungen fand sich eine gute Korrelation(More)
In-vitro sensitivity to cystostatic agents was tested in 137 cases of breast carcinoma, with 125 tests being analysable. Inhibition of in-vitro incorporation of 3H-uridine or 3H-thymidine into RNA or DNA of the original tumour cells after incubation with antineoplastic substances was used as the index for the in-vivo sensitivity of the tumour. 30% of the(More)
The essential Bacillus anthracis nrdE gene carries a self-splicing group I intron with a putative homing endonuclease belonging to the GIY-YIG family. Here, we show that the nrdE pre-mRNA is spliced and that the homing endonuclease cleaves an intronless nrdE gene 5 nucleotides (nt) upstream of the intron insertion site, producing 2-nt 3' extensions. We also(More)
Several group I introns have been previously found in strains of the Bacillus cereus group at three different insertion sites in the nrdE gene of the essential nrdIEF operon coding for ribonucleotide reductase. Here, we identify an uncharacterized group IA intron in the nrdF gene in 12 strains of the B. cereus group and show that the pre-mRNA is efficiently(More)
strahlensensibel, dab der Versuch bereits nach einer Gesamtdosis von 3000 rad abgebrochen werden konnte. Histologiseh war der bestrahlte Tumor tiberwiegend nekrotisch bis auf einzelne monstrSse Zellen. Der freiwerdende Raum wurde dureh hyalinisierte Substanzen und Bindegewebe eingenommen. Wie kein anderes in vitro-Modell scheinen sieh thymusaplastische(More)