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Most metazoan microRNAs (miRNAs) target many genes for repression, but the nematode lsy-6 miRNA is much less proficient. Here we show that the low proficiency of lsy-6 can be recapitulated in HeLa cells and that miR-23, a mammalian miRNA, also has low proficiency in these cells. Reporter results and array data indicate two properties of these miRNAs that(More)
Most metazoan microRNAs (miRNAs) target many genes for repression, but the nematode lsy-6 miRNA is much less proficient. Here, we show that the low proficiency of lsy-6 can be recapitulated in HeLa cells and that miR-23 (a mammalian miRNA) also has low proficiency in these cells. Reporter results and array data both indicate two properties of these miRNAs(More)
Prions are a paradigm-shifting mechanism of inheritance in which phenotypes are encoded by self-templating protein conformations rather than nucleic acids. Here, we examine the breadth of protein-based inheritance across the yeast proteome by assessing the ability of nearly every open reading frame (ORF; ∼5,300 ORFs) to induce heritable traits. Transient(More)
We evaluate different call admission control policies in various mul-tiservice cellular scenarios. For each of the studied policies we obtain the maximum calling rate that can be offered to the system to achieve a given QoS objective defined in terms of blocking probabilities. We propose an optimization methodology based on a hill climbing algorithm to find(More)
The objective of this paper 1 is to provide a robust representation of moving images based on layers. To that goal, we have designed eecient motion estimation and segmentation techniques by aane model tting suitable for the construction of layers. Layered representations, originally introduced in 4] are important in several applications. In particular, they(More)
This paper presents a text-based emotion identification system based on text implemented by means of a language-independent architecture. The system includes several natural language processing tasks besides an affective keyword dictionary. The main novelty of the system is the incorporation of a semantic disambi-guation module which focuses on the meaning(More)
A 20S proteasome, comprising two subunits alpha and beta, was purified from the extreme halophilic archaeon Haloarcula marismortui, which grows only in saturated salt conditions. The three-dimensional reconstruction of the H. marismortui proteasome (Hm proteasome), obtained from negatively stained electron micrographs, is virtually identical to the(More)
Prions are proteins that convert between structurally and functionally distinct states, at least one of which is self-perpetuating. The prion fold templates the conversion of native protein, altering its structure and function, and thus serves as a protein-based element of inheritance. Molecular chaperones ensure that these prion aggregates are divided and(More)