Julia Gorelik12
James McColl8
Steven F. Lee7
Yuri E Korchev7
Mathew H. Horrocks7
12Julia Gorelik
8James McColl
7Steven F. Lee
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DNA sequence information underpins genetic research, enabling discoveries of important biological or medical benefit. Sequencing projects have traditionally used long (400-800 base pair) reads, but the existence of reference sequences for the human and many other genomes makes it possible to develop new, fast approaches to re-sequencing, whereby shorter(More)
  • Nunilo Cremades, Samuel I.A. Cohen, Emma Deas, Andrey Y. Abramov, Allen Y. Chen, Angel Orte +9 others
  • 2012
Here, we use single-molecule techniques to study the aggregation of α-synuclein, the protein whose misfolding and deposition is associated with Parkinson's disease. We identify a conformational change from the initially formed oligomers to stable, more compact proteinase-K-resistant oligomers as the key step that leads ultimately to fibril formation. The(More)
  • David Lando, Ulrike Endesfelder, Harald Berger, Lakxmi Subramanian, Paul D. Dunne, James McColl +6 others
  • 2012
The inheritance of the histone H3 variant CENP-A in nucleosomes at centromeres following DNA replication is mediated by an epigenetic mechanism. To understand the process of epigenetic inheritance, or propagation of histones and histone variants, as nucleosomes are disassembled and reassembled in living eukaryotic cells, we have explored the feasibility of(More)
We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique(More)
  • Yu Ye, Georg Blaser, Mathew H. Horrocks, Maria J. Ruedas-Rama, Shehu Ibrahim, Alexander A. Zhukov +4 others
  • 2012
Mechanisms of protein recognition have been extensively studied for single-domain proteins, but are less well characterized for dynamic multidomain systems. Ubiquitin chains represent a biologically important multidomain system that requires recognition by structurally diverse ubiquitin-interacting proteins. Ubiquitin chain conformations in isolation are(More)
Single molecule tracking is widely used to monitor the change in position of lipids and proteins in living cells. In many experiments in which molecules are tagged with a single or small number of fluorophores, the signal/noise ratio may be limiting, the number of molecules is not known, and fluorophore blinking and photobleaching can occur. All these(More)
Advances in imaging and microscopy have driven many discoveries in biology over the past century. Electron-micro-scopy-based techniques (for example, freeze-fracture, freeze-etching, and/or immunogold labeling [1]) have provided a wealth of evidence that cell plasma membranes are organized into structural and functional microdomains. [2] However, these(More)
Oligomers of the 40 and 42 residue amyloid-β peptides (Aβ40 and Aβ42) have been implicated in the neuronal damage and impaired cognitive function associated with Alzheimer's disease. However, little is known about the specific mechanisms by which these misfolded species induce such detrimental effects on cells. In this work, we use single-molecule imaging(More)
The boundary between the physical and biological sciences has been eroded in recent years with new physical methods applied to biology and biological molecules being used for new physical purposes. We have pioneered the application of a form of scanning probe microscopy based on a scanned nanopipette, originally developed by Hansma and co-workers, for(More)
Understanding the component stoichiometry of the T cell antigen receptor (TCR) triggering apparatus is essential for building realistic models of signal initiation. Recent studies suggesting that the TCR and other signaling-associated proteins are preclustered on resting T cells relied on measurements of the behavior of membrane proteins at interfaces with(More)