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We hypothesized that stearoyl-CoA desaturase (SCD) enzyme activity would not correlate with fatty acid indices of SCD activity in steers fed different grains. Forty-five Angus steers (358 +/- 26 kg BW) were individually fed for 107 d diets differing in whole cottonseed (WCS) supplementation (0, 5, or 15% of DM) and grain source (rolled corn, flaxseed plus(More)
In this study, the interactions among breed of cattle, adipose tissue site and specific incubation conditions were investigated. Subcutaneous and i.m. adipose tissues were obtained from 10 Angus and 9 Santa Gertrudis steers immediately postmortem. Adipose tissue explants were incubated acutely for 2 h immediately at slaughter or after being cultured 48 h(More)
Angus (n = 10) and crossbred (3 4 and 7 8 ) Wagyu (n = 10) steers were fed a diet according to typical Japanese standards for 552 days. The steers were fed to gain approximately 0·90 kg/head/day. Fatty acid composition was determined for subcutaneous and intramuscular adipose tissue, and M. longissimus dorsi muscle. Trained sensory evaluation and a(More)
This investigation addressed the hypothesis that stearoyl coenzyme A desaturase (SCD) gene expression would serve as a postnatal marker of adipocyte differentiation in bovine s.c. adipose tissue. Samples of tailhead s.c. adipose tissue were obtained by biopsy from preweaning steer calves 2.5 wk, 5 mo, and 7.5 mo of age and from yearling steers 12 mo of age.(More)
The effects of a high oleate sunflower seed diet on tissue composition and on fatty acid elongation and desaturation enzyme activities were investigated. Three Simmental calves were fed a standard corn-based diet (2.6% fat); three others were fed the corn-based diet containing 20% high oleate sunflower seed (10.4% fat). Blood samples and perianal adipose(More)
Forty-five Angus steers (avg initial wt 330 kg) were individually fed for 112 d to assess the value of supplemental Zn and source on performance and carcass quality. Steers had ad libitum access to a control diet (81 ppm Zn) of 33% whole corn, 33% ground milo, 15% cottonseed hulls and 13% cottonseed meal, or this control diet with 360 mg Zn/d added from(More)
We hypothesized that the concentrations of monounsaturated fatty acids (MUFA) and cholesterol of adipose tissue and M. longissimus thoracis would not differ between Angus and American Wagyu steers when fed to a typical US live weight, but would diverge when fed to a Japanese live weight. To test this, 8 steers of each breed type were assigned to a(More)
The primary objective of this study was to provide evidence for preadipocyte proliferation during culture of adipose tissue explants; a secondary objective was to compare the lipogenic activity and cellularity of adipose tissues from American Wagyu crossbred steers. Subcutaneous (s.c.) and intramuscular (i.m.) adipose tissues were obtained at slaughter from(More)
Effects of breed type and sex on the fatty acid composition of subcutaneous neutral lipid and intramuscular neutral and phospholipids of longissimus lumborum muscle were investigated using 145 steers and 82 heifers that consisted of pure Japanese Black and Holstein and crossbreds among Japanese Black, Holstein, Japanese Brown, and Charolais. Steers and(More)
Ten purebred Angus and ten crossbred (3/4-7/8) American Wagyu steers were fed a corn and barley-based diet for 552 days and slaughtered. All USDA and Japanese yield and quality grade factors were evaluated and the amount of extractable lipid and moisture in totally trimmed muscle was determined. Angus steers gained 0·9 kg/head/day and American Wagyus gained(More)