Alzheimer-type neuropathology in transgenic mice overexpressing V717F β-amyloid precursor protein
Transgenic mice that express high levels of human mutant APP support a primary role for APP/Aβ in the genesis of AD and could provide a preclinical model for testing therapeutic drugs.
The permeability of the endplate channel to organic cations in frog muscle
The relative permeability of endplate channels to many organic cations was determined by reversal-potential criteria and specific chemical factors seem to be less important than access or friction in determining the ionic selectivity of the endplate channel.
Novel ω-Conotoxins from Conus catus Discriminate among Neuronal Calcium Channel Subtypes*
In electrophysiological studies, ω-conotoxins CVID and MVIIA had similar potencies to inhibit current through central and peripheral splice variants of the rat N-type calcium channels when coexpressed with rat β3 in Xenopus oocytes, but the potency of CVID increased when α1B-d and α1b-b were expressed in the absence of ratβ3.
The permeability of endplate channels to monovalent and divalent metal cations
Permeability ratios for divalent ions decreased as the concentration of divalent ion was increased in a manner consistent with the negative surface potential theory of Lewis (1979 J. Physiol.
Ion channels and regulation of intracellular calcium in vascular endothelial cells
Oscillations in [Ca2+]i, which may involve both Ca2+ entry and release, have been observed in isolated and confluent endothelial cell monolayers stimulated by histamine and bradykinin, and are consistent with a cycle that involves the plasma membrane.
μO-conotoxin MrVIB selectively blocks Nav1.8 sensory neuron specific sodium channels and chronic pain behavior without motor deficits
- J. Ekberg, A. Jayamanne, R. Lewis
- BiologyProceedings of the National Academy of Sciences
- 7 November 2006
It is demonstrated that μO-conotoxin MrVIB from Conus marmoreus displays substantial selectivity for Nav1.8 and inhibits pain behavior in models of persistent pain and can alleviate chronic pain behavior with a greater therapeutic index than nonselective antagonists.
Regulation of Neuronal Voltage-gated Sodium Channels by the Ubiquitin-Protein Ligases Nedd4 and Nedd4-2*
- Andrew B. Fotia, J. Ekberg, David John Adams, D. Cook, P. Poronnik, Sharad Kumar
- BiologyJournal of Biological Chemistry
- 1 July 2004
It is shown that the carboxyl termini fusion proteins of one central nervous system and one peripheral nervous system-derived Na+ channel are readily ubiquitinated by Nedd4-2, and these channels have conserved WW domain binding specificity.
Sleeping Beauty mutagenesis reveals cooperating mutations and pathways in pancreatic adenocarcinoma
- K. Mann, J. Ward, N. Copeland
- Biology, MedicineProceedings of the National Academy of Sciences
- 15 March 2012
Sleeping Beauty mutagenesis provides a rich resource of mutations in potential cancer drivers for cross-comparative analyses with ongoing sequencing efforts in human pancreatic adenocarcinoma.
Voltage‐dependent sodium and calcium currents in cultured parasympathetic neurones from rat intracardiac ganglia.
The kinetics of activation and inactivation of the Ca2+ current were voltage dependent and the time course of inactivation was fitted by the sum of two exponentials, which increased at depolarized membrane potentials.
The Doublecortin-Expressing Population in the Developing and Adult Brain Contains Multipotential Precursors in Addition to Neuronal-Lineage Cells
Results indicate that DCXhigh cells, regardless of location, are restricted to the neuronal lineage or are bone fide neurons, whereas some DCXlow cells retain their multipotentiality.