Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage
It is shown that a double cut HDR donor, which is flanked by single guide RNA (sgRNA)-PAM sequences and is released after CRISPR/Cas9 cleavage, increases HDR efficiency by twofold to fivefold relative to circular plasmid donors.
The importance of vitamin d metabolism as a potential prophylactic, immunoregulatory and neuroprotective treatment for COVID-19
The potential role of vitamin D extra-renal metabolism in the prevention and treatment of the SARS-CoV-2 infection is summarized, helping to bring us slightly closer to fulfilling that goal.
Comparison of the biological actions of TGF beta‐1 and TGF beta‐2: Differential activity in endothelial cells
- J. Jennings, S. Mohan, T. Linkhart, Richard Widstrom, David J. Baylink
- Biology, MedicineJournal of Cellular Physiology
- 1 October 1988
Inhibition of DNA synthesis in endothelial cells is relatively specific for TGF beta‐1, and both TGFbeta‐1 ad TGF Beta‐2 are potent mitogens for chicken embryonic calvarial bone cells.
Efficient Reprogramming of Human Cord Blood CD34+ Cells Into Induced Pluripotent Stem Cells With OCT4 and SOX2 Alone.
It is shown that the lentiviral expression of OCT4 together with SOX2 (OS) driven by a strong spleen focus-forming virus (SFFV) promoter in a single vector can convert 2% of CB CD34+ cells into iPSCs without additional reprogramming factors.
Efficient Generation of Integration-Free iPS Cells from Human Adult Peripheral Blood Using BCL-XL Together with Yamanaka Factors
It is shown that Yamanaka factors (OCT4, SOX2, MYC, and KLF4)-expressing EV can also reprogram adult peripheral blood mononuclear cells (PBMNCs) into pluripotency, yet at a very low efficiency.
DNA Microarrays: Their Use and Misuse
This review focuses on uncertainty areas of microarray technology that include making microarrays, isolation of RNA and labeling, hybridization and scanning, and data analysis and briefly summarizes microarray's applications in biomedical research.
Highly efficient genome editing via CRISPR–Cas9 in human pluripotent stem cells is achieved by transient BCL-XL overexpression
It is reported that the transient delivery of BCL-XL increases iPSC survival by ∼10-fold after plasmid transfection, leading to a 20- to 100-fold increase in homology-directed repair (HDR) KI efficiency and a 5- fold increase in non-homologous end joining (NHEJ) KO efficiency.
Patient-specific cardiovascular progenitor cells derived from integration-free induced pluripotent stem cells for vascular tissue regeneration.
PDGFB-based stem cell gene therapy increases bone strength in the mouse
- Wanqiu Chen, David J. Baylink, Xiao-Bing Zhang
- Biology, MedicineProceedings of the National Academy of Sciences
- 6 July 2015
It is found that modest overexpression of PDGFB using a relatively weak phosphoglycerate kinase (PGK) promoter completely avoided osteomalacia and secondary hyperparathyroidism, and simultaneously increased trabecular bone formation and trABecular connectivity, and decreased cortical porosity, resulting in a large increase in bone strength.
1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.
The data support 2 conclusions: (a) M1 macrophages are important for the recruitment and osteogenic priming of MSCs and, hence, are necessary for fracture repair, and (b) under vitamin D-sufficient conditions, 1,25(OH)2D treatment is unnecessary and can be detrimental if provided during the proinflammatory stage of fracture healing.