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This study tested KUROKURA solution (Kurokura et al., 1984, Aquaculture 37, 267–274) and its modifications (by increasing NaCl content to 160, 180 and 200 mM) on immobilizing properties for sampling and short-term preservation of potential motility of tench spermatozoa. The immobilizing solution is used because, when collected, the sperm of most samples is(More)
Various procedures for artificial insemination in tench, Tinca tinca (L.) were re-examined with evaluation of fecundity of males and females among different tench strains. The objectives of this study were to enhance fertilization and hatching rates through optimization of the activation solution, the insemination process, the activation of gametes, and the(More)
The effect of cryopreservation on the protein phosphorylation/dephosphorylation pattern of common carp (Cyprinus carpio) sperm is described. Sperm was diluted in dimethyl sulfoxide (DMSO) and ethylene glycol (EG)-based extenders, followed by equilibration, freezing, and thawing. Proteins extracted from fresh and cryopreserved spermatozoa were separated on(More)
The spermiation of tench males was stimulated with Supergestran containing mammalian LHRHa lecireline at the following doses: 5, 10, 20 and 40  $$\upmu$$ g kg−1 b.w.; then with carp pituitary suspension (CPS) at a dose of 2 mg kg−1 b.w. and with a control of saline physiological solution. The following days, meaning 24, 48 and 72 h after injection, sperm(More)
A basic assumption for carrying out the breeding work is to have well-organised broodstock management. Taking into account the number of populations bred and the limited number of ponds available, it is impossible to avoid rearing several juvenile or adult breeds in mixed stocks. Fish of individual breeds are group-marked with regular renewal of the(More)
The percentage of sperm motility (92-100%), spermatozoan velocity (112-163 microm.s(-1)) and control hatching rates (83-96%) were evaluated for each of six gold and five green male common carp (Cyprinus carpio). In all 30 possible paired combinations of sperm-competition tests, hatching rates of 90-97% were achieved. The mean percentage of offspring sired(More)
Northern pike (Esox lucius L.) spermatozoa are uniflagellated cells differentiated into a head without acrosome, a midpiece and a flagellar tail region flanked by a fin structure. Total, flagellar, head and midpiece lengths of spermatozoa were measured and show mean values of 34.5, 32.0, 1.32, 1.17 microm, respectively, with anterior and posterior widths of(More)
The present study shows the roles of osmolality, calcium (Ca(2+))-potassium (K(+)) antagonist and Ca(2+) in sperm activation and flagellar beating of a sturgeon species, sterlet (Acipenser ruthenus). Sperm motility was activated at hypoosmolality relative to seminal plasma and suppressed at 175 mOsmol kg(-1). Sperm activation was totally suppressed by(More)
This study summarizes optimization of techniques for common carp artificial propagation including improvements of activation solution (AS), the process of insemination, and elimination of egg stickiness. The optimum gamete ratio for good fertilization and hatching rate ranged from 8490 to 23 672 spermatozoa per egg, when dechlorinated tap water was used.(More)
In the present study was summarize the factors activating and/or inhibiting the motility of intact and/or demembranated spermatozoa of common carp and paddlefish as teleostean and chondrostean models using methods of sperm demembranation. The movement of spermatozoa of cyprinids species, common carp, is influenced by osmotic pressure or high concentration(More)