David G. Crider

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Self-renewing embryonic stem (ES) cells have been established from early mouse embryos as permanent cell lines. By cultivation in vitro as three-dimensional aggregates called embryoid bodies (EBs), ES cells can differentiate into derivatives of all three primary germ layers, including cardiomyocytes. ES cells thus represent a useful model system for(More)
The Chk2-mediated deoxyribonucleic acid (DNA) damage checkpoint pathway is important for mitochondrial DNA (mtDNA) maintenance. We show in this paper that mtDNA itself affects cell cycle progression. Saccharomyces cerevisiae rho(0) cells, which lack mtDNA, were defective in G1- to S-phase progression. Deletion of subunit Va of cytochrome c oxidase,(More)
BACKGROUND The transcription factor B-Myb is present in all proliferating cells, and in mice engineered to remove this gene, embryos die in utero just after implantation due to inner cell mass defects. This lethal phenotype has generally been attributed to a proliferation defect in the cell cycle phase of G1. METHODOLOGY/PRINCIPAL FINDINGS In the present(More)
Embryonic stem (ES) cell lines represent a population of undifferentiated pluripotent cells capable of multilineage differentiation in vitro. Although very useful for studying developmental processes, human ES cell lines have also been suggested as a potential and unlimited source for cellular transplantation and the treatment of human disease. The(More)
Movement and positional control of mitochondria and other organelles are coordinated with cell cycle progression in the budding yeast, Saccharomyces cerevisiae. Recent studies have revealed a checkpoint that inhibits cytokinesis when there are severe defects in mitochondrial inheritance. An established checkpoint signaling pathway, the mitotic exit network(More)
Mitochondrial inheritance, the transfer of mitochondria from mother to daughter cell during cell division, is essential for daughter cell viability. The mitochore, a mitochondrial protein complex containing Mdm10p, Mdm12p, and Mmm1p, is required for mitochondrial motility leading to inheritance in budding yeast. We observe a defect in cytokinesis in(More)
D.G. Crider and L.J. García-Rodríguez contributed equally to this paper. Correspondence to Liza A. Pon: lap5@columbia.edu L.J. García-Rodríguez’s present address is Paterson Institute for Cancer Research, Cancer Research UK, University of Manchester, Manchester M20 4BX, England, UK. Abbreviations used in this paper: ATM, ataxia telangiectasia mutated; dNTP,(More)
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