David E. Ott

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HIV-1 particle production is driven by the Gag precursor protein Pr55(Gag). Despite significant progress in defining both the viral and cellular determinants of HIV-1 assembly and release, the trafficking pathway used by Gag to reach its site of assembly in the infected cell remains to be elucidated. The Gag trafficking itinerary in primary monocyte-derived(More)
— We consider the problem of applying aggregate congestion control to a class of distributed multi-media applications known as Cluster-to-Cluster (C-to-C) applications. Flows in such an application share a common intermediary path that is the primary source of network delay and packet loss. Using the Coordination Protocol (CP) architecture, we show how(More)
HIV-1 integrase (IN) is a virally encoded protein required for integration of viral cDNA into host chromosomes. INI1/hSNF5 is a component of the SWI/SNF complex that interacts with HIV-1 IN, is selectively incorporated into HIV-1 (but not other retroviral) virions, and modulates multiple steps, including particle production and infectivity. To gain further(More)
We consider the problem of flow coordination in distributed multimedia applications. Most transport-level protocols are designed to operate independently and lack mechanisms for sharing information with other flows and coordinating data transport in various ways. This limitation becomes problematic in distributed applications that employ numerous flows(More)
— In this paper, we identify an emerging and important application class comprised of a set of processes on a cluster of devices communicating to a remote set of processes on another cluster of devices across a common intermediary Internet path. We call these applications cluster-to-cluster applications, or C-to-C applications. The networking requirements(More)
The presence of relatively high levels of cellular protein contamination in density-purified virion preparations is a confounding factor in biochemical analyses of HIV and SIV produced from hematopoietic cells. A major source of this contamination is from vesicles, either microvesicles or exosomes, that have similar physical properties as virions. Thus,(More)
SHP, the capsid-stabilizing protein of lambdoid phage 21, is highly resistant against denaturant-induced unfolding. We demonstrate that this high functional stability of SHP is due to a high kinetic stability with a half-life for unfolding of 25 days at zero denaturant, while the thermodynamic stability is not unusually high. Unfolding experiments(More)
BACKGROUND The HIV-1 p6 Gag protein regulates the final abscission step of nascent virions from the cell membrane by the action of two late assembly (L-) domains. Although p6 is located within one of the most polymorphic regions of the HIV-1 gag gene, the 52 amino acid peptide binds at least to two cellular budding factors (Tsg101 and ALIX), is a substrate(More)