David C. Chapman

Learn More
The expression of proteins using recombinant baculoviruses is a mature and widely used technology. However, some aspects of the technology continue to detract from high throughput use and the basis of the final observed expression level is poorly understood. Here, we describe the design and use of a set of vectors developed around a unified cloning strategy(More)
OBJECTIVE To evaluate the accuracy, clinical correlation, ease of use, and patient acceptance of the Coaguchek and the ProTime Microcoagulation System as compared with standard laboratory methods for prothrombin time determination. METHODS A total of 30 prothrombin times, expressed as international normalized ratios (INRs), were determined by each(More)
In this work, we modified our wafer-scale 3D integration technique, originally developed for Si, to hybridize InP-based image sensor arrays with Si readout circuits. InGaAs image arrays based on the InGaAs layer grown on InP substrates were fabricated in the same processing line as silicon-on-insulator (SOI) readout circuits. The finished 150-mm-diameter(More)
3H-Muscimol, a structural analog of y-aminobutyric acid (GABA) , binds to rat brain synaptic membranes in a saturable manner, with a high affinity dissociation constant of 2.2 nM. Binding is increased in membranes that have been treated with Triton X-1Q0 and is not 3 dependent upon sodium. High affinity H-muscimol binding is regionally distributed, with(More)
Two test methods, a skin replica test and the Kelsey Sykes test have been used to compare the efficacy of three antiseptics against three Gram-negative micro-organisms in an attempt to select the most suitable. The results of both tests showed that an antiseptic containing added ethylenediaminetetraacetic acid was superior to the other two. The skin test(More)
Information on the accumulation and/or depletion of Zn in metallothionein (MT) of rat fetus, rat pup, and maternal rat liver at various ages was obtained with pregnant rats fed a basal casein diet or this diet plus either 100 ppm Zn or 50 ppm Cd. Rats fed each of the respective diets were sacrificed on 12, 16, and 20 d of gestation and 0, 7, 14, and 28 d(More)
  • 1