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RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations in the genomes ofExpand
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Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system
The ability to artificially control transcription is essential both to the study of gene function and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA-guidedExpand
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Cas9 specifies functional viral targets during CRISPR-Cas adaptation
Clustered regularly interspaced short palindromic repeat (CRISPR) loci and their associated (Cas) proteins provide adaptive immunity against viral infection in prokaryotes. Upon infection, shortExpand
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CRISPR interference can prevent natural transformation and virulence acquisition during in vivo bacterial infection.
Pathogenic bacterial strains emerge largely due to transfer of virulence and antimicrobial resistance genes between bacteria, a process known as horizontal gene transfer (HGT). Clustered, regularlyExpand
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PhageTerm: a tool for fast and accurate determination of phage termini and packaging mechanism using next-generation sequencing data
The worrying rise of antibiotic resistance in pathogenic bacteria is leading to a renewed interest in bacteriophages as a treatment option. Novel sequencing technologies enable description of anExpand
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Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials
Antibiotics target conserved bacterial cellular pathways or growth functions and therefore cannot selectively kill specific members of a complex microbial population. Here, we develop programmable,Expand
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Conjugative DNA Transfer Induces the Bacterial SOS Response and Promotes Antibiotic Resistance Development through Integron Activation
Conjugation is one mechanism for intra- and inter-species horizontal gene transfer among bacteria. Conjugative elements have been instrumental in many bacterial species to face the threat ofExpand
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Consequences of Cas9 cleavage in the chromosome of Escherichia coli
The RNA-guided Cas9 nuclease from CRISPR-Cas systems has emerged as a powerful biotechnological tool. The specificity of Cas9 can be reprogrammed to cleave desired sequences in a cell's chromosomeExpand
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Conditional tolerance of temperate phages via transcription-dependent CRISPR-Cas targeting
A fundamental feature of immune systems is the ability to distinguish pathogenic from self and commensal elements, and to attack the former but tolerate the latter. Prokaryotic CRISPR-Cas immuneExpand
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Adapting to new threats: the generation of memory by CRISPR‐Cas immune systems
Clustered, regularly interspaced, short palindromic repeats (CRISPR) loci and their associated genes (cas) confer bacteria and archaea with adaptive immunity against phages and other invading geneticExpand
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