David A. T. Somers

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Integration of transgenic DNA into the plant genome was investigated in 13 transgenic oat (Avena sativa L.) lines produced using microprojectile bombardment with one or two cotransformed plasmids. In all transformation events, the transgenic DNA integrated into the plant genome consisted of intact transgene copies that were accompanied by multiple,(More)
Soybean is a major crop species providing valuable feedstock for food, feed and biofuel. In recent years, considerable progress has been made in developing genomic resources for soybean, including on-going efforts to sequence the genome. These efforts have identified a large number of soybean genes, most with unknown function. Therefore, a major research(More)
Maize (Zea mays, cv 'Black Mexican Sweet') (BMS) and tobacco (Nicotiana tabacum, cv 'Xanthi') tissue cultures were transformed using silicon carbide fibers to deliver DNA into suspension culture cells. DNA delivery was mediated by vortexing cells in the presence of silicon carbide fibers and plasmid DNA. Maize cells were treated with a plasmid carrying both(More)
An irregular pattern of transgene silencing was revealed in expression and inheritance studies conducted over multiple generations following transgene introduction by microprojectile bombardment of allohexaploid cultivated oat (Avena sativa L.). Expression of two transgenes, bar and uidA, delivered on the same plasmid was investigated in 23 transgenic oat(More)
AtMYB44, a member of the subgroup 22 R2R3 MYB transcription factors, positively regulates abscisic acid signaling to induce stomatal closure, thus conferring drought/salt-stress tolerance in Arabidopsis thaliana. In this study, AtMYB44 was transformed into soybean [Glycine max (L.) Merrill] using the cotyledonary-node method. The resulting homozygous lines(More)
Enhanced aluminum (Al) resistance has been observed in dicots over-expressing enzymes involved in organic acid synthesis; however, this approach for improving Al resistance has not been investigated in monocots. Among the cereals, oat (Avena sativa L.) is considered to be Al resistant, but the basis of resistance is not known. A hydroponic assay and(More)
Measurements of the Casimir force require the elimination of the electrostatic force between the surfaces. However, due to electrostatic patch potentials, the voltage required to minimize the total force may not be sufficient to completely nullify the electrostatic interaction. Thus, these surface potential variations cause an additional force, which can(More)
Crop plant transformation systems are typically labor intensive and involve highly repetitive tasks. This presentation will cover strategies for and experiences with applying automation to transgenic plant pipelines with the aim of increasing system throughput and capacity while reducing labor and costs associated with production of transgenic plants.(More)
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