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  • D A Caron
  • 1983
A new method is described that uses the fluorochrome primulin and epifluorescence microscopy for the enumeration of heterotrophic and phototrophic nanoplankton (2 to 20 mum). Phototrophic microorganisms are distinguished from heterotrophs by the red autofluorescence of chlorophyll a. Separate filter sets are used which allow visualization of the(More)
Protistan diversity was characterized at three locations in the western North Atlantic (Sargasso Sea and Gulf Stream) by sequencing 18S rRNA genes in samples from euphotic (< or = 125 m) and bathypelagic depths (2500 m). A total of 923 partial-length protistan sequences were analysed, revealing 324 distinct operational taxonomic units (OTUs) determined by(More)
The diversity of protistan assemblages has traditionally been studied using microscopy and morphological characterization, but these methods are often inadequate for ecological studies of these communities because most small protists inherently lack adequate taxonomic characters to facilitate their identification at the species level and many protistan(More)
Microplankton abundances and phytoplankton mortality rates were determined at six stations during four cruises spanning three seasons in the Ross Sea polynya, Antarctica (early spring, Oct.}Nov. 1996; mid-late summer, Jan.}Feb. 1997; fall, Apr. 1997; mid-late spring, Nov.}Dec. 1997). Rates of microzooplankton herbivory were measured using a modi"ed dilution(More)
Phototrophic and heterotrophic nanoplankton (PNAN, HNAN; 2–20 mm protists) and microplankton (PMIC, HMIC; 20–200 mm protists and micrometazoa) are major taxa involved in partitioning carbon and energy within the pelagic food web. In the Ross Sea, Antarctica, plankton biomass appears to be controlled by the seasonal recession of the sea ice and the formation(More)
Ostreococcus is a genus of widely distributed marine phytoplankton which are picoplanktonic in size (<2 mum) and capable of rapid growth. Although Ostreococcus has been detected around the world, little quantitative information exists on its contribution to planktonic communities. We designed and implemented a genus-specific TaqMan-based quantitative PCR(More)
Photosynthetic dinoflagellates contain a diverse collection of plastid types, a situation believed to have arisen from multiple endosymbiotic events. In addition, a number of heterotrophic (phagotrophic) dinoflagellates possess the ability to acquire chloroplasts temporarily by engulfing algae and retaining their chloroplasts in a functional state. These(More)
single-celled, microscopic forms nearly 4 billion years ago. These minute forms constituted all life on the planet for roughly half of Earth's biological history, and microbes have remained important determinants of organic matter production , trophic transfer, and degradation throughout Earth's entire history, although more attention and research tend to(More)
A fluorescent in situ hybridization method that uses rRNA-targeted oligonucleotide probes for counting protists in cultures and environmental water samples is described. Filtration, hybridization, and enumeration of fixed cells with biotinylated eukaryote-specific probes and fluorescein isothiocyanate-conjugated avidin were performed directly on(More)