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Phylogenetic Analysis of Hemoplasma Species: an International Study
ABSTRACT Nearly complete 16S rRNA gene sequences for feline and canine hemoplasma isolates from Europe, Australia, Africa, and Asia showed almost 100% identity to those previously reported for UnitedExpand
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Use of Real-Time PCR To Detect and Quantify Mycoplasma haemofelis and “Candidatus Mycoplasma haemominutum” DNA
ABSTRACT A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis and “Candidatus Mycoplasma haemominutum” in feline blood samples. The assay was rapid andExpand
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The application of polymerase chain reaction to the detection of rotaviruses in faeces.
An assay protocol based on exploiting the polymerase chain reaction (PCR) for the detection of rotavirus in infected faeces is described. The assay is 100,000 times more sensitive than the standardExpand
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Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR.
A real-time reverse transcription polymerase chain reaction assay was developed to detect feline calicivirus infection in cats. Using SYBR green I melting curve analysis, isolates could beExpand
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Detection of Chlamydophila felis and Feline Herpesvirus by Multiplex Real-Time PCR Analysis
ABSTRACT Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allowExpand
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Molecular biology of rotaviruses. IX. Conservation and divergence in genome segment 5.
Nucleotide sequencing of RNA segment 5 from seven strains of group A rotavirus has been carried out to investigate the extent of diversity and conservation, as well as possible selective pressuresExpand
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Responses of cats to nasal vaccination with a live, modified feline herpesvirus type 1.
Intranasal vaccination with a cold-adapted strain of feline herpesvirus type 1 (FHV-1) two days before challenge gave partial protection, and four days before challenge gave complete protection,Expand
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Use of a Taqman PCR to determine the response of Mycoplasma haemofelis infection to antibiotic treatment.
A quantitative Taqman polymerase chain reaction (PCR) assay was used to evaluate the response of Mycoplasma haemofelis experimentally infected cats to three antibiotic treatment regimes. Sixteen catsExpand
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Detection of nucleotide polymorphisms in feline calicivirus isolates by reverse transcription PCR and a fluorescence resonance energy transfer probe.
A fluorescence resonance energy transfer system has been developed to detect nucleotide polymorphisms in isolates of feline calicivirus (FCV). Isolates with an exact match to the 28 nucleotide probeExpand
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Molecular typing of strains of staphylococcus aureus isolated from bone and joint lesions in lame broilers by random amplification of polymorphic DNA.
A study was conducted to investigate the relationship between the presence of localized bacterial infection and lameness in broiler fowl (Gallus gallus domesticus). Isolation of bacteria from theExpand
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