Darwin K. Berg

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1993). Eight are designated as ␣-type (␣2–␣9) and three Center for Neurobiology and Behavior as ␤-type genes (␤2–␤4; also called non-␣1–␣3). Assign-College of Physicians and Surgeons ment to the neuronal ␣ subunit group requires inclusion Columbia University of the adjacent cysteine residues equivalent to amino New York, New York 10032 acids 192 and 193 of(More)
Nicotinic acetylcholine receptors containing the alpha7 gene product are expressed at substantial levels in the hippocampus. Because of their specific locations and their high relative calcium permeability, the receptors not only mediate cholinergic transmission in the hippocampus but also influence signaling at noncholinergic synapses. We have used(More)
Nicotinic receptors are cation-ion selective ligand-gated ion channels that are expressed throughout the nervous system. Most have significant calcium permeabilities, enabling them to regulate calcium-dependent events. One of the most abundant is a species composed of the alpha 7 gene product and having a relative calcium permeability equivalent to that of(More)
Seguela et al., 1993). Activation of the receptors can produce a rapidly decaying inward current (Zorumski et al., 1992; Alkondon and Albuquerque, 1993; Zhang et Summary al., 1994) and can quickly elevate intracellular levels of Nicotinic acetylcholine receptors are widely distribfree calcium in neurons (Vijayaraghavan et al., 1992). In uted throughout the(More)
GABA (gamma-aminobutyric acid), the major inhibitory transmitter in the brain, goes through a transitory phase of excitation during development. The excitatory phase promotes neuronal growth and integration into circuits. We show here that spontaneous nicotinic cholinergic activity is responsible for terminating GABAergic excitation and initiating(More)
Neurotransmitter release is well known to occur at specialized synaptic regions that include presynaptic active zones and postsynaptic densities. At cholinergic synapses in the chick ciliary ganglion, however, membrane formations and physiological measurements suggest that release distant from postsynaptic densities can activate the predominantly(More)
Synaptic activation of the transcription factor CREB and downstream gene expression usually depend on calcium influx aided by voltage-gated calcium channels. We find that nicotinic signaling, in contrast, activates CREB and gene expression in ciliary ganglion neurons both in culture and in situ only if voltage-gated channels are silent. The nicotinic(More)
Protein scaffolds are essential for specific and efficient downstream signaling at synapses. Though nicotinic receptors are widely expressed in the nervous system and influence numerous cellular events due in part to their calcium permeability, no scaffolds have yet been identified for the receptors in neurons. Here we show that specific members of the(More)
Nicotinic receptors containing alpha7 subunits are ligand-gated ion channels widely distributed in the nervous system; they influence a diverse array of events because of their high relative calcium permeability. We show here that nicotine-induced whole-cell responses generated by such receptors can be dramatically potentiated in a rapidly reversible manner(More)
Activity-dependent gene expression is essential for form and function in the nervous system. Best understood is the role of glutamatergic signaling in controlling such events, but nicotinic signaling can also regulate transcription. We show here that nicotine can alter gene expression in rat hippocampal neurons, as reflected by activation of the(More)